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The immune response is interrelated to the progress of anhedonia and despair symptoms of <jats:italic>T. gondii<\/jats:italic>-infected subjects. We recently reported that serum <jats:italic>N<\/jats:italic>-glycans were altered in mice displayed depressive-like behaviors. However, a novel biomarker that correlated to <jats:italic>T. gondii<\/jats:italic> infection and associated behaviors is demanded. Glycomics has been used to find affected glycoproteins during depression. The objective of this study is to investigate serum <jats:italic>N<\/jats:italic>-glycomics changes during infection with <jats:italic>T. gondii<\/jats:italic> in BALB\/c mice, immunocompetent, or in severe combined immunodeficient mice, and after treatment with an immunostimulant; 1-methyl tryptophan. Glycans were examined through glycoblotting-protocol then investigated by MALDI-TOF\/MS. Both depressive and sickness-related behaviors were significantly abundant (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.001 each), during acute <jats:italic>T. gondii<\/jats:italic> in immunocompetent mice, compared to controls. Only sickness symptoms were evident in immunodeficient mice infected with <jats:italic>T. gondii<\/jats:italic>, as associated with high expression level (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.001) of Peak # 15 (2\u2009\u00d7\u2009Neu5Gc) compared to controls. The alteration of sialylated <jats:italic>N<\/jats:italic>-glycan expressions is important to detect the immune status of animals\/humans against <jats:italic>T. gondii<\/jats:italic>. Moreover, 1-methyl tryptophan reduced depressive-like behavior (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.001) compared to controls. Therefore, sialylated <jats:italic>N<\/jats:italic>-glycan (Neu5Ac\/Neu5Gc-terminal) is targeted to be used as a novel biomarker of sickness\/depressive-like behaviors.<\/jats:p>","DOI":"10.1038\/s41598-020-60681-4","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T11:03:15Z","timestamp":1583146995000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sialylated N-glycan profile during acute and chronic infections with Toxoplasma gondii in mice"],"prefix":"10.1038","volume":"10","author":[{"given":"Ibrahim Farag","family":"Rehan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motamed Elsayed","family":"Mahmoud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doaa","family":"Salman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asmaa","family":"Elnagar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saleh","family":"Salman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Youssef","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amer Ragheb Abdel","family":"Aziz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman Kamal","family":"Bazh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abd El-Latif","family":"Hesham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,3,2]]},"reference":[{"key":"60681_CR1","doi-asserted-by":"crossref","unstructured":"Mahmoud, M. 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