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Calcium and copper ions were encapsulated in biopolymeric microparticles (microspheres and microcapsules) either as single agents or in combination with biocontrol agents, <jats:italic>Trichoderma viride<\/jats:italic> spores, a fungal plant growth mediator. Both, calcium and copper ions are directly involved in the synthesis of plant secondary metabolites and alongside, <jats:italic>Trichoderma viride<\/jats:italic> can provide indirect stimulation and higher uptake of nutrients. All treatments with microparticles had a positive effect on the enhancement of plant secondary metabolites content in <jats:italic>Lactuca sativa<\/jats:italic> L. The highest increase of chlorophylls, antioxidant activity and phenolic was obtained by calcium-based microparticles in both, conventionally and hydroponically grown lettuces. Non-encapsulated fungus <jats:italic>Trichoderma viride<\/jats:italic> enhanced the synthesis of plant secondary metabolites only in hydroponics cultivation signifying the importance of its encapsulation. 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