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Currently, the only therapy available is invasive and there is a known recurrence after surgery. In this study, we tested the inhibitory and amyloid dissociation properties of four osmolytes in an <jats:italic>in-vitro<\/jats:italic><jats:italic>TGFBI<\/jats:italic> peptide aggregation model. The 23-amino acid long peptide (TGFBIp 611\u2013633 with the mutation c.623\u2009G&gt;R) from the 4th FAS-1 domain of TGFBIp that rapidly forms amyloid fibrils was used in the study. Several biophysical methods like Thioflavin T (ThT) fluorescence, Circular Dichroism (CD), fluorescence microscopy and Transmission electron microscopy (TEM) were used to study the inhibitory and amyloid disaggregation properties of the\u00a0four osmolytes (Betaine, Raffinose, Sarcosine, and Taurine). The osmolytes were effective in both inhibiting and disaggregating the\u00a0amyloid fibrils derived from TGFBIp 611\u2013633\u2009c.623\u2009G&gt;R peptide. The osmolytes did not have an adverse toxic effect on cultured human corneal fibroblast cells and could potentially be a useful therapeutic strategy for patients with TGFBIp corneal dystrophies.<\/jats:p>","DOI":"10.1038\/s41598-020-60944-0","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T11:02:30Z","timestamp":1583319750000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Effect of osmolytes on in-vitro aggregation properties of peptides derived from TGFBIp"],"prefix":"10.1038","volume":"10","author":[{"given":"Anandalakshmi","family":"Venkatraman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elavazhagan","family":"Murugan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu Jun","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary Swee Lim","family":"Peh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshminarayanan","family":"Rajamani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jodhbir S.","family":"Mehta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,3,4]]},"reference":[{"issue":"3","key":"60944_CR1","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1038\/nrd3050","volume":"9","author":"A Aguzzi","year":"2010","unstructured":"Aguzzi, A. & O\u2019Connor, T. 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