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Here, we report the first analysis of heart transcriptome variation using microarrays in an AGAT-deficient (AGAT<jats:sup>\u2212\/\u2212<\/jats:sup>) mouse model to evaluate AGAT-, creatine- and HA-dependent gene regulation. Our data revealed significant differences of gene expression between AGAT<jats:sup>\u2212\/\u2212<\/jats:sup> and wild-type (WT) mice, affecting cardiac energy metabolism (<jats:italic>Fbp2<\/jats:italic>, <jats:italic>Ucp2<\/jats:italic>), cardiac hypertrophy and fibrosis (<jats:italic>Nppa<\/jats:italic>, <jats:italic>Ctgf<\/jats:italic>), immune response (<jats:italic>Fgl2<\/jats:italic>), and the conduction system of the heart (<jats:italic>Dsc2<\/jats:italic>, <jats:italic>Ehd4<\/jats:italic>, <jats:italic>Hcn2, Hcn4<\/jats:italic>, <jats:italic>Scn4a, Scn4b<\/jats:italic>). All of these genes being expressed on WT level in creatine-supplemented mice. Using <jats:italic>in silico<\/jats:italic> analysis based on the GEO database we found that most of these candidate genes (<jats:italic>Ctgf<\/jats:italic>, <jats:italic>Dsc2<\/jats:italic>, <jats:italic>Fbp2<\/jats:italic>, <jats:italic>Fgl2<\/jats:italic>, <jats:italic>Hcn2<\/jats:italic>, <jats:italic>Nppa<\/jats:italic>) \u00a0revealed significant alterations in a WT\u00a0mouse model of myocardial infarction underlining a pathophysiological relationship between AGAT metabolism and cardiovascular disease.<\/jats:p>","DOI":"10.1038\/s41598-020-61638-3","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T11:05:42Z","timestamp":1584356742000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of L-arginine:glycine amidinotransferase-, creatine- and homoarginine-dependent gene regulation in the murine heart"],"prefix":"10.1038","volume":"10","author":[{"given":"M\u00e4rit","family":"Jensen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-un","family":"Choe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edzard","family":"Schwedhelm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Zeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,16]]},"reference":[{"key":"61638_CR1","doi-asserted-by":"publisher","first-page":"3232","DOI":"10.1093\/eurheartj\/ehw334","volume":"37","author":"N Townsend","year":"2016","unstructured":"Townsend, N. et al. 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The other authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"4821"}}