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In this study, we found that oxidative damage induced by endogenous reactive oxygen species (ROS) plays an important role in the antifungal activity of BN-3b. Further investigation indicated that BN-3b stimulated ROS accumulation, increased malondialdehyde (MDA) levels, and decreased reduced\/oxidized glutathione (GSH\/GSSG) ratio. Moreover, BN-3b decreased mitochondrial membrane potential (MMP) and ATP generation. Ultrastructure analysis revealed that BN-3b severely damaged the cell membrane of <jats:italic>C. albicans<\/jats:italic>. Quantitative PCR (RT-qPCR) analysis revealed that virulence factors of <jats:italic>C. albicans SAP<\/jats:italic>s, <jats:italic>PLB1<\/jats:italic>, <jats:italic>PLB2<\/jats:italic>, <jats:italic>HWP1<\/jats:italic>, <jats:italic>ALS<\/jats:italic>s, and <jats:italic>LIP<\/jats:italic>s were all down-regulated after BN-3b exposure. We also found that BN-3b markedly inhibited the hyphal formation of <jats:italic>C. albicans<\/jats:italic>. In addition, <jats:italic>in vivo<\/jats:italic> studies revealed that BN-3b significantly prolonged survival and decreased fungal burden in mouse model of disseminated candidiasis.<\/jats:p>","DOI":"10.1038\/s41598-020-61681-0","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T11:04:07Z","timestamp":1584615847000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A semisynthetic borrelidin analogue BN-3b exerts potent antifungal activity against Candida albicans through ROS-mediated oxidative damage"],"prefix":"10.1038","volume":"10","author":[{"given":"Hao","family":"Su","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caijuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bixuan","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodan","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peipei","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Mu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2780-0978","authenticated-orcid":false,"given":"Li","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1561-8108","authenticated-orcid":false,"given":"Xueshi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,19]]},"reference":[{"key":"61681_CR1","doi-asserted-by":"publisher","first-page":"414","DOI":"10.3389\/fcimb.2017.00414","volume":"7","author":"M Zawrotniak","year":"2017","unstructured":"Zawrotniak, M. et al. 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