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While there is a\u00a0considerable degree of genetic diversity amongst isolates,\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    has a robust global biogeographic structure and genetic populations are spatially clustered in the environment. We examined the distribution and local spread\u00a0of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    in Darwin, Northern Territory, Australia, which has the highest recorded urban incidence of melioidosis globally. We sampled soil and land runoff throughout the city centre and performed whole-genome sequencing (WGS)\u00a0on\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    isolates. By combining phylogenetic analyses, Bayesian clustering and spatial hot spot analysis our results demonstrate that some sequence types (STs) are widespread in the urban Darwin environment, while others are highly spatially clustered over a small geographic scale. This clustering matches the spatial distribution of clinical cases for one ST. Results also demonstrate a greater overall isolate diversity recovered from drains compared to park soils, further supporting the role drains may play in dispersal of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    STs in the environment. Collectively, knowledge gained from this study will allow for better understanding of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    phylogeography and melioidosis source attribution, particularly on a local level.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-62300-8","type":"journal-article","created":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T07:03:32Z","timestamp":1585119812000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Whole-genome sequencing of Burkholderia pseudomallei from an urban melioidosis hot\u00a0spot reveals a fine-scale population structure and localised spatial clustering in the environment"],"prefix":"10.1038","volume":"10","author":[{"given":"Audrey","family":"Rachlin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Mayo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica R.","family":"Webb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Kleinecke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Rigas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenda","family":"Harrington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart J.","family":"Currie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjam","family":"Kaestli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,25]]},"reference":[{"key":"62300_CR1","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1128\/CMR.18.2.383-416.2005","volume":"18","author":"AC Cheng","year":"2005","unstructured":"Cheng, A. 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