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Over the last few years, second harmonic generation (SHG) microscopy has been used for the analysis of collagen fibers in several types of gynaecological cancers, such as breast and ovarian cancer. The value of collagen parameters obtained with this technique to gain insights on the physiopathology and on the prognostic evaluation of cancer has been advocated. Herein, we have characterized the collagen fibers in squamous cell carcinoma (VSCC) and preneoplastic lesions using the SHG microscopy. Collagen parameters, quantity, organization, and uniformity, of VSCC,\u00a0adjacent skin of VSCC or preneoplastic lesions were compared with values obtained in\u00a0normal tissue of healthy control. There was an evident decrease in the values of collagen fiber parameters in the VSCC. Increased quantity and uniformity of tumor associated collagen fibers were associated with the presence of lymph node metastases, which suggest a prognostic value of such parameters in the evaluation of vulvar cancer.<\/jats:p>","DOI":"10.1038\/s41598-020-62346-8","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T11:03:11Z","timestamp":1585306991000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Study on collagen parameters in vulvar cancer and preneoplastic lesions by Second Harmonic Generation microscopy"],"prefix":"10.1038","volume":"10","author":[{"given":"Maria das Gra\u00e7as de F\u00e1tima Cavalcan","family":"Castor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leuridan Cavalcante","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Jos\u00e9 Vieira de","family":"Mello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo de Andrade","family":"Natal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Vassallo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,3,27]]},"reference":[{"key":"62346_CR1","doi-asserted-by":"crossref","unstructured":"Wang, Z. et al. 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