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Here, we characterize a whole-body mouse knockout of\n                    <jats:italic>Gpr27<\/jats:italic>\n                    .\n                    <jats:italic>Gpr27<\/jats:italic>\n                    knockout mice were born at expected Mendelian ratios and exhibited no gross abnormalities. Insulin and\n                    <jats:italic>Pdx1<\/jats:italic>\n                    mRNA in\n                    <jats:italic>Gpr27<\/jats:italic>\n                    knockout islets were reduced by 30%, but this did not translate to a reduction in islet insulin content or beta cell mass.\n                    <jats:italic>Gpr27<\/jats:italic>\n                    knockout mice exhibited slightly worsened glucose tolerance with lower plasma insulin levels while maintaining similar insulin tolerance. Unexpectedly,\n                    <jats:italic>Gpr27<\/jats:italic>\n                    deletion reduced expression of\n                    <jats:italic>Eif4e3<\/jats:italic>\n                    , a neighboring gene, likely by deleting transcription start sites on the anti-sense strand of the\n                    <jats:italic>Gpr27<\/jats:italic>\n                    coding exon. Our data confirm that loss of\n                    <jats:italic>Gpr27<\/jats:italic>\n                    reduces insulin mRNA\n                    <jats:italic>in vivo<\/jats:italic>\n                    but has only minor effects on glucose tolerance.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-62358-4","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T07:03:11Z","timestamp":1585292591000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Deletion of Gpr27 in vivo reduces insulin mRNA but does not result in diabetes"],"prefix":"10.1038","volume":"10","author":[{"given":"Deeksha G.","family":"Chopra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Yiv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas G.","family":"Hennings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaohuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5306-4225","authenticated-orcid":false,"given":"Gregory M.","family":"Ku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,27]]},"reference":[{"key":"62358_CR1","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1038\/nrd.2017.178","volume":"16","author":"AS Hauser","year":"2017","unstructured":"Hauser, A. 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