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In particular, the pseudostratified neuroepithelia of the retina and cerebrocortical ventricular zones provide a platform for progenitor cell proliferation and migration. Lamination defects in these tissues lead to mispositioned neurons, disrupted neuronal connections, and abnormal function. The molecular mechanisms necessary for proper lamination in these tissues are incompletely understood. Here, we identified a nonsense mutation in the <jats:italic>Eml1<\/jats:italic> gene in a novel murine model, <jats:italic>tvrm360<\/jats:italic>, displaying subcortical heterotopia, hydrocephalus and disorganization of retinal architecture. In the retina, <jats:italic>Eml1<\/jats:italic> disruption caused abnormal positioning of photoreceptor cell nuclei early in development. Upon maturation, these ectopic photoreceptors possessed cilia and formed synapses but failed to produce robust outer segments, implying a late defect in photoreceptor differentiation secondary to mislocalization. In addition, abnormal positioning of M\u00fcller cell bodies and bipolar cells was evident throughout the inner neuroblastic layer. Basal displacement of mitotic nuclei in the retinal neuroepithelium was observed in <jats:italic>tvrm360<\/jats:italic> mice at postnatal day 0. The abnormal positioning of retinal progenitor cells at birth and ectopic presence of photoreceptors and secondary neurons upon maturation suggest that EML1 functions early in eye development and is crucial for proper retinal lamination during cellular proliferation and development.<\/jats:p>","DOI":"10.1038\/s41598-020-62373-5","type":"journal-article","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T11:03:21Z","timestamp":1585307001000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Disruption in murine Eml1 perturbs retinal lamination during early development"],"prefix":"10.1038","volume":"10","author":[{"given":"G. 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