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The major cellular adhesin, P1, localises to the tip of the attachment organelle forming a complex with P40 and P90, two cleavage fragments derived by processing Mpn142, and other molecules with adhesive and mobility functions. LC-MS\/MS analysis of <jats:italic>M<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> M129 proteins derived from whole cell lysates and eluents from affinity matrices coupled with chemically diverse host molecules identified 22 proteoforms of P1. Terminomics was used to characterise 17 cleavage events many of which were independently verified by the identification of semi-tryptic peptides in our proteome studies and by immunoblotting. One cleavage event released <jats:sup>1597<\/jats:sup>TSAAKPGAPRPPVPPKPGAPKPPVQPPKKPA<jats:sup>1627<\/jats:sup> from the C-terminus of P1 and this peptide was shown to bind to a range of host molecules. A smaller synthetic peptide comprising the C-terminal 15 amino acids, <jats:sup>1613<\/jats:sup>PGAPKPPVQPPKKPA<jats:sup>1627<\/jats:sup>, selectively bound cytoskeletal intermediate filament proteins cytokeratin 7, cytokeratin 8, cytokeratin 18, and vimentin from a native A549 cell lysate. Collectively, our data suggests that ectodomain shedding occurs on the surface of <jats:italic>M<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> where it may alter the functional diversity of P1, Mpn142 and other surface proteins such as elongation factor Tu via a mechanism similar to that described in <jats:italic>Mycoplasma hyopneumoniae<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41598-020-63136-y","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T10:03:12Z","timestamp":1586858592000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Cell surface processing of the P1 adhesin of Mycoplasma pneumoniae identifies novel domains that bind host molecules"],"prefix":"10.1038","volume":"10","author":[{"given":"Michael","family":"Widjaja","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iain James","family":"Berry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronica Maria","family":"Jarocki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew Paul","family":"Padula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Dumke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven Philip","family":"Djordjevic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,4,14]]},"reference":[{"key":"63136_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1574-6968.2001.tb10610.x","volume":"198","author":"DC Krause","year":"2001","unstructured":"Krause, D. 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