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In tumours, high metabolic rate leads to acidosis affecting cancer progression. Acidic pH affects activities of remodelling cells <jats:italic>in vitro<\/jats:italic>. The pH measurement predicts healing in pathological wounds and success of surgical treatment of burns and chronic ulcers. However, current methods are limited to skin surface or based on detection of fluorescence intensity of specific sensitive probes that suffer of microenvironment factors. Herein, we ascertained relevance <jats:italic>in vivo<\/jats:italic> of cell metabolic adaptation in skin repair by interfering with anaerobic glycolysis. Moreover, a custom-designed skin imaging chamber, 2-Photon microscopy (2PM), fluorescence lifetime imaging (FLIM) and data mapping analyses were used to correlate maps of glycolytic activity <jats:italic>in vivo<\/jats:italic> as measurement of NADH intrinsic lifetime with areas of hypoxia and acidification in models of skin injury and cancer. The method was challenged by measuring the NADH profile by interfering with anaerobic glycolysis and oxidative phosphorylation in the mitochondrial respiratory chain. Therefore, intravital NADH FLIM represents a tool for investigating cell metabolic adaptation occurring in wounds, as well as the relationship between cell metabolism and cancer.<\/jats:p>","DOI":"10.1038\/s41598-020-63203-4","type":"journal-article","created":{"date-parts":[[2020,4,14]],"date-time":"2020-04-14T10:02:56Z","timestamp":1586858576000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Evaluation of cell metabolic adaptation in wound and tumour by Fluorescence Lifetime Imaging Microscopy"],"prefix":"10.1038","volume":"10","author":[{"given":"Diego","family":"Morone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca D\u2019","family":"Autilia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilo","family":"Schorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Erreni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Doni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,4,14]]},"reference":[{"key":"63203_CR1","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1016\/S0006-3495(02)73936-2","volume":"83","author":"KM Hanson","year":"2002","unstructured":"Hanson, K. 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