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To estimate the antibiotic resistance and molecular characteristics of <jats:italic>S. aureus<\/jats:italic> causing BSIs in Shanghai, 120\u00a0<jats:italic>S. aureus<\/jats:italic> isolates (20 isolates each year) from the patients with <jats:italic>S. aureus<\/jats:italic> BSIs from 2013 to 2018 were randomly selected and enrolled in this study. Fifty-three (44.2%) MRSA isolates were determined, and no isolate was found resistant to vancomycin, daptomycin, synercid, linezolid and ceftaroline. The toxin genes <jats:italic>tst<\/jats:italic>, <jats:italic>sec<\/jats:italic>, <jats:italic>seg<\/jats:italic> and <jats:italic>sei<\/jats:italic> were found more frequently among MRSA isolates compared with MSSA isolates (all <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). Twenty-nine sequence types (STs) were identified, and ST5 (23.3%) was the most common ST, followed by ST398 (11.7%) and ST764 (10.0%). SCC<jats:italic>mec<\/jats:italic> II (73.6%) was the most frequent SCC<jats:italic>mec<\/jats:italic> type among MRSA isolates. The dominant clonal complexes (CCs) were CC5 (ST5, ST764, ST965 and ST3066; 36.7%) and the livestock-associated clone CC398 (ST398, 11.7%). MRSA-CC5 was the predominant CC among MRSA isolates (37\/53, 69.8%), and CC5-II MRSA was found in 34 isolates accounting for 91.9% (34\/37) among CC5 MRSA isolates. In addition, all 29 <jats:italic>tst<\/jats:italic>-positive MRSA isolates were CC5-MRSA as well. Our study provided the properties and genotypes of <jats:italic>S. aureus<\/jats:italic> causing BSIs at Ruijin Hospital in Shanghai from 2013 to 2018, and might suggest of value clues for the further study insights into pathogenic mechanisms intrinsically referring to the development of human-adapted <jats:italic>S. aureus<\/jats:italic> clones and their diffusions.<\/jats:p>","DOI":"10.1038\/s41598-020-63248-5","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T10:02:45Z","timestamp":1586253765000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus Causing Bloodstream Infections at Ruijin Hospital in Shanghai from 2013 to 2018"],"prefix":"10.1038","volume":"10","author":[{"given":"Feifei","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhen","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxing","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhong","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"63248_CR1","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1111\/1469-0691.12144","volume":"19","author":"KB Laupland","year":"2013","unstructured":"Laupland, K. 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