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Methods: Antimicrobial susceptibility testing was performed using Kirby-Bauer disc diffusion system. Quinolone resistance-determining region (QRDR), plasmid-mediated quinolone resistance (PMQR) determinant genes were detected by PCR and sequencing. Results: Out of 239 <jats:italic>Salmonella<\/jats:italic> isolates, 164 were <jats:italic>S<\/jats:italic>. Typhi and 75 were <jats:italic>S<\/jats:italic>. Paratyphi. 128 (53.6%) <jats:italic>Salmonella<\/jats:italic> isolates were resistant to nalidixic acid; 11 (4.6%) isolates to ciprofloxacin and 66 (27.6%) isolates were intermediate to ciprofloxacin. QRDR were present in 69\u2009<jats:italic>S<\/jats:italic>. Typhi isolates, among which mutation at codon 83 (<jats:italic>n<\/jats:italic>\u2009=\u200945) and 133 (<jats:italic>n<\/jats:italic>\u2009=\u200961) predominated. In <jats:italic>S<\/jats:italic>. Paratyphi, the most common mutations were detected in <jats:italic>gyrA<\/jats:italic> at codon 83(<jats:italic>n\u2009<\/jats:italic>=\u200924) and <jats:italic>parC<\/jats:italic>: T57S (<jats:italic>n<\/jats:italic>\u2009=\u20098). Seven mutations were first reported in <jats:italic>Salmonella<\/jats:italic> isolates including <jats:italic>gyrB<\/jats:italic>: S426G, <jats:italic>parC<\/jats:italic>: D79G and <jats:italic>parE<\/jats:italic>: [S498T, E543K, V560G, I444S, Y434S]. PMQR genes including <jats:italic>qnrD1<\/jats:italic>, <jats:italic>qnrA1<\/jats:italic>, <jats:italic>qnrB4<\/jats:italic>, <jats:italic>aac (6<\/jats:italic>\u2032<jats:italic>)-Ib-cr4<\/jats:italic> and <jats:italic>qnrS1<\/jats:italic> were detected in 1, 2, 3, 7\u00a0and 9 isolates, relatively. Conclusions: High resistance to quinolones in <jats:italic>Salmonella<\/jats:italic> remains a serious problem in Jiangsu, China. The presence of the novel mutations increases the complexity of quinolone-resistant genotypes and poses a threat to public health. Subject terms: <jats:italic>Salmonella<\/jats:italic> Typhi, <jats:italic>Salmonella<\/jats:italic> Paratyphi, antimicrobial resistance, QRDR, PMQR.<\/jats:p>","DOI":"10.1038\/s41598-020-64346-0","type":"journal-article","created":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T10:02:59Z","timestamp":1588240979000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Discovery of seven novel mutations of gyrB, parC and parE in Salmonella Typhi and Paratyphi strains from Jiangsu Province of China"],"prefix":"10.1038","volume":"10","author":[{"given":"Huimin","family":"Qian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoye","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongming","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Hong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dazhi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjun","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,4,30]]},"reference":[{"key":"64346_CR1","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1086\/649541","volume":"50","author":"JA Crump","year":"2010","unstructured":"Crump, J. 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