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In Northern Europe, LNB is primarily caused by the spirochete <jats:italic>Borrelia garinii<\/jats:italic> and transmitted by the tick <jats:italic>Ixodes ricinus<\/jats:italic>. This Danish observational epidemiologic case-control study includes every identified LNB patient (n\u2009=\u2009401) on Funen, Denmark, from 1995-2014. We display spatial and temporal LNB incidence variation, seasonal distribution of cases and local spatial case clustering. Seasonal patterns show LNB symptom-onset peaking in July and a significant seasonal difference in number of cases (p\u2009&lt;\u20090.01). We found no significant change in seasonality patterns over time when dividing the study period into 5-year intervals. We identified a significant local geographical hot-spot of cases with a relative risk of 2.44 (p\u2009=\u20090.013). Analysis revealed a significantly shorter distance to nearest forest for cases compared with controls (p\u2009&lt;\u20090.001). We present a novel map of the focal geographical distribution of LNB cases in a high endemic borreliosis area. Continued studies of case clustering in the epidemiology of LNB are of key importance in guiding intervention strategies.<\/jats:p>","DOI":"10.1038\/s41598-020-64638-5","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T10:03:33Z","timestamp":1588932213000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Spatial and temporal patterns of Lyme Neuroborreliosis on Funen, Denmark from 1995\u20132014"],"prefix":"10.1038","volume":"10","author":[{"given":"Amalie Muus","family":"Andreasen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petter Bart","family":"Dehlendorff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrikke Christie","family":"Knudtzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene","family":"B\u00f8dker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene Jung","family":"Kj\u00e6r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigurdur","family":"Skarphedinsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,8]]},"reference":[{"key":"64638_CR1","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1080\/003655400458857","volume":"32","author":"P Jensen","year":"2000","unstructured":"Jensen, P., Hansen, H. & Frandsen, F. 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