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Here, we developed a rapid, specific and sensitive visual detection of <jats:italic>sea<\/jats:italic> using loop-mediated isothermal amplification (LAMP) combined with nanogold probe (AuNP) or styryl dye (STR). LAMP-AuNP and LAMP-STR can detect as low as 9.7 fg (3.2 <jats:italic>sea<\/jats:italic> copies) and 7.2 <jats:italic>sea<\/jats:italic> copies, respectively, which were lower than PCR (97 fg or 32 <jats:italic>sea<\/jats:italic> copies). The excellent performance of these new assays was demonstrated in food samples using crude DNA lysates. While the culture method detected 10<jats:sup>4<\/jats:sup> CFU\/g in ground pork and 10 CFU\/mL in milk in 5\u20137 days, LAMP-AuNP could detect down to 10 CFU\/g for both samples in 27\u2009minutes. Analyzing 80 pork and milk samples revealed that the LAMP-AuNP showed 100% sensitivity, 97\u2013100% specificity and 97.5\u2013100% accuracy, which were superior to the culture method, and comparable to PCR but without requirement of a thermal cycler. Furthermore, our LAMP-AuNP detect <jats:italic>sea<\/jats:italic> at a range below the food safety control (&lt;100 CFU\/g). The LAMP-STR quantitated <jats:italic>sea<\/jats:italic> in 10\u20131,000 CFU (7.2\u2013720 copies). Our crude DNA lysis combined with LAMP-AuNP\/STR present effective point-of-care detection and facilitate appropriate control strategies.<\/jats:p>","DOI":"10.1038\/s41598-020-64710-0","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T10:03:33Z","timestamp":1588932213000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Rapid colorimetric loop-mediated isothermal amplification for hypersensitive point-of-care Staphylococcus aureus enterotoxin A gene detection in milk and pork products"],"prefix":"10.1038","volume":"10","author":[{"given":"Grittaya","family":"Srimongkol","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boonsong","family":"Ditmangklo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilada","family":"Choopara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiraporn","family":"Thaniyavarn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Dean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirirat","family":"Kokpol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tirayut","family":"Vilaivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naraporn","family":"Somboonna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,8]]},"reference":[{"key":"64710_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0168-1605(00)00377-9","volume":"61","author":"N Balaban","year":"2000","unstructured":"Balaban, N. & Rasooly, A. 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