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Here, we aim to investigate the importance of <jats:italic>S. aureus<\/jats:italic> Lpp and TLR2 in a hematogenous septic arthritis model, which is the most common route of infection in humans. C57BL\/6 wild-type and TLR2 deficient mice were intravenously inoculated with <jats:italic>S. aureus<\/jats:italic> Newman parental strain or its lipoprotein-deficient <jats:italic>\u0394lgt<\/jats:italic> mutant strain. The clinical course of septic arthritis, radiological changes, and serum levels of cytokines and chemokines, were assessed. Newman strain induced more severe and frequent clinical septic polyarthritis compared to its <jats:italic>\u0394lgt<\/jats:italic> mutant in TLR2 deficient mice, but not in wild-type controls. Bone destruction, however, did not differ between groups. Lpp expression was associated with higher mortality, weight loss as well as impaired bacterial clearance in mouse kidneys independent of TLR2. Furthermore, Lpp expression induced increased systemic pro-inflammatory cytokine and neutrophil chemokine release. Staphylococcal Lpp are potent virulence factors in <jats:italic>S. aureus<\/jats:italic> systemic infection independent of host TLR2 signalling. However, they have a limited impact on bone erosion in hematogenous staphylococcal septic arthritis.<\/jats:p>","DOI":"10.1038\/s41598-020-64879-4","type":"journal-article","created":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T10:02:50Z","timestamp":1589364170000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The role of Staphylococcus aureus lipoproteins in hematogenous septic arthritis"],"prefix":"10.1038","volume":"10","author":[{"given":"Majd","family":"Mohammad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhicheng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abukar","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep Kumar","family":"Kopparapu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manli","family":"Na","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Jarneborn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana do Nascimento","family":"Stroparo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minh-Thu","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Karlsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich","family":"G\u00f6tz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rille","family":"Pullerits","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,13]]},"reference":[{"key":"64879_CR1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1186\/1678-9199-20-19","volume":"20","author":"PM Colavite","year":"2014","unstructured":"Colavite, P. M. & Sartori, A. Septic arthritis: immunopathogenesis, experimental models and therapy. J. Venom. Anim. Toxins Incl. Trop. Dis. 20, 19, https:\/\/doi.org\/10.1186\/1678-9199-20-19 (2014).","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"64879_CR2","doi-asserted-by":"publisher","first-page":"1819","DOI":"10.1002\/art.1780381215","volume":"38","author":"CJ Kaandorp","year":"1995","unstructured":"Kaandorp, C. J., Van Schaardenburg, D., Krijnen, P., Habbema, J. D. & van de Laar, M. A. Risk factors for septic arthritis in patients with joint disease. A prospective study. Arthritis Rheum. 38, 1819\u20131825 (1995).","journal-title":"Arthritis Rheum."},{"key":"64879_CR3","doi-asserted-by":"publisher","first-page":"1810","DOI":"10.1136\/ard.2011.152769","volume":"70","author":"JB Galloway","year":"2011","unstructured":"Galloway, J. B. et al. Risk of septic arthritis in patients with rheumatoid arthritis and the effect of anti-TNF therapy: results from the British Society for Rheumatology Biologics Register. Ann. Rheum. Dis. 70, 1810\u20131814, https:\/\/doi.org\/10.1136\/ard.2011.152769 (2011).","journal-title":"Ann. Rheum. Dis."},{"key":"64879_CR4","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1016\/j.berh.2006.08.001","volume":"20","author":"A Tarkowski","year":"2006","unstructured":"Tarkowski, A. Infection and musculoskeletal conditions: Infectious arthritis. Best. Pract. Res. Clin. Rheumatol. 20, 1029\u20131044, https:\/\/doi.org\/10.1016\/j.berh.2006.08.001 (2006).","journal-title":"Best. Pract. Res. Clin. Rheumatol."},{"key":"64879_CR5","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1136\/ard.56.8.470","volume":"56","author":"CJ Kaandorp","year":"1997","unstructured":"Kaandorp, C. J. et al. Incidence and sources of native and prosthetic joint infection: a community based prospective survey. 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