{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T21:50:07Z","timestamp":1787003407224,"version":"build-2736575974"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study aimed to investigate whether interleukin-34 (\n                    <jats:italic>IL-34<\/jats:italic>\n                    ) mRNA expression is aberrant and modulated by tumor necrosis factor-alpha (TNF-\u03b1) in knee osteoarthritis (OA) fibroblast-like synoviocytes (FLS) and determine associations of IL-34 mRNA and protein in the systemic and local joint environments with severity of knee OA synovitis. Transcriptional and translational IL-34 levels in FLS, synovium, synovial fluid, and plasma of knee OA were determined using real-time polymerase chain reaction, immunohistochemistry, and enzyme-linked immunosorbent assay. Relative mRNA expressions of NF-\u03baB signaling molecules were further measured. In knee OA FLS stimulated with TNF-\u03b1,\n                    <jats:italic>IL-34<\/jats:italic>\n                    mRNA expression was significantly up-regulated in a time-dependent manner. In knee OA synovium with severe synovitis, increased\n                    <jats:italic>IL-34<\/jats:italic>\n                    mRNA expression was directly associated with\n                    <jats:italic>IL-6<\/jats:italic>\n                    ,\n                    <jats:italic>I\u03baB<\/jats:italic>\n                    ,\n                    <jats:italic>NF-\u03baB<\/jats:italic>\n                    , and\n                    <jats:italic>MMP-13<\/jats:italic>\n                    , in addition to knee OA FLS. Immunostaining score of IL-34 was considerably greater in knee OA synovium with severe synovitis than that in those with mild and no synovitis. Increments in joint fluid and plasma IL-34 levels in knee OA patients with severe synovitis were closely related to its mRNA and protein expressions in knee OA synovium. Transcriptional and translational expressions of IL-34 were positively correlated with synovitis severity. Collectively, IL-34 overexpression would reflect synovitis severity in knee OA.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-64932-2","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T06:03:18Z","timestamp":1589436198000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Interleukin-34 overexpression mediated through tumor necrosis factor-alpha reflects severity of synovitis in knee osteoarthritis"],"prefix":"10.1038","volume":"10","author":[{"given":"Wanvisa","family":"Udomsinprasert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artit","family":"Jinawath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nipaporn","family":"Teerawattanapong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sittisak","family":"Honsawek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,14]]},"reference":[{"key":"64932_CR1","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1016\/j.apmr.2011.11.029","volume":"93","author":"MW Creaby","year":"2012","unstructured":"Creaby, M. 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