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Here we identified the differentially expressed genes (DEGs) of circulating lymphocytes and monocytes to reveal potential biomarkers that may be used as molecular targets for future diagnosis of each combination of these pathologies (compared to healthy patients) and give insights into the underlying molecular mechanisms of these diseases. Study participants (n\u2009=\u2009150) were divided into groups: (H) systemically and periodontal healthy (control group); (P) with periodontitis, but systemically healthy; (DL-P) with dyslipidemia and periodontitis; (T2DMwell-DL-P) well-controlled type 2 diabetes mellitus with dyslipidemia and periodontitis; and (T2DMpoorly-DL-P) poorly-controlled type 2 diabetes mellitus with dyslipidemia and periodontitis. We preprocessed the microarray data using the Robust Multichip Average (RMA) strategy, followed by the RankProd method to identify candidates for DEGs. Furthermore, we performed functional enrichment analysis using Ingenuity Pathway Analysis and Gene Set Enrichment Analysis. DEGs were submitted to pairwise comparisons, and selected DEGs were validated by quantitative polymerase chain reaction. Validated DEGs verified from T2DMpoorly-DL-P <jats:italic>versus<\/jats:italic> H were: <jats:italic>TGFB1I1<\/jats:italic>, <jats:italic>VNN1<\/jats:italic>, <jats:italic>HLADRB4<\/jats:italic> and <jats:italic>CXCL8<\/jats:italic>; T2DMwell-DL-P <jats:italic>versus<\/jats:italic> H: <jats:italic>FN1<\/jats:italic>, <jats:italic>BPTF<\/jats:italic> and <jats:italic>PDE3B<\/jats:italic>; DL-P <jats:italic>versus<\/jats:italic> H: <jats:italic>DAB2<\/jats:italic>, <jats:italic>CD47<\/jats:italic> and <jats:italic>HLADRB4<\/jats:italic>; P <jats:italic>versus<\/jats:italic> H: <jats:italic>IGHDL-P<\/jats:italic>, <jats:italic>ITGB2<\/jats:italic> and <jats:italic>HLADRB4<\/jats:italic>. In conclusion, we identified that circulating lymphocytes and monocytes of individuals simultaneously affected by T2DM, dyslipidemia and periodontitis, showed an altered molecular profile mainly associated to inflammatory response, immune cell trafficking, and infectious disease pathways. Altogether, these results shed light on novel potential targets for future diagnosis, monitoring or development of targeted therapies for patients sharing these conditions.<\/jats:p>","DOI":"10.1038\/s41598-020-65042-9","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T10:02:50Z","timestamp":1589796170000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Circulating lymphocytes and monocytes transcriptomic analysis of patients with type 2 diabetes mellitus, dyslipidemia and periodontitis"],"prefix":"10.1038","volume":"10","author":[{"given":"S\u00e2mia C. 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