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Our study aimed at elucidating 11p15.5 3D structure, via 3C and 3D FISH analyses of cell lines derived from healthy, BWS or SRS children. We found that, in healthy cells, <jats:italic>IGF2\/H19<\/jats:italic> and <jats:italic>CDKN1C\/KCNQ1OT1<\/jats:italic> domains fold in complex chromatin conformations, that facilitate the control of IGs mediated by distant enhancers. In patient-derived cell lines, we observed a profound impairment of such a chromatin architecture. Specifically, we identified a cross-talk between <jats:italic>IGF2\/H19<\/jats:italic> and <jats:italic>CDKN1C\/KCNQ1OT1<\/jats:italic> domains, consisting in <jats:italic>in cis<\/jats:italic>, monoallelic interactions, that are present in healthy cells but lost in patient cell lines: an inter-domain association that sees ICR2 move close to <jats:italic>IGF2<\/jats:italic> on one allele, and to <jats:italic>H19<\/jats:italic> on the other. Moreover, an intra-domain association within the <jats:italic>CDKN1C\/KCNQ1OT1 locus<\/jats:italic> seems to be crucial for maintaining the 3D organization of the region.<\/jats:p>","DOI":"10.1038\/s41598-020-65082-1","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T10:04:11Z","timestamp":1589882651000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Profound alterations of the chromatin architecture at chromosome 11p15.5 in cells from Beckwith-Wiedemann and Silver-Russell syndromes patients"],"prefix":"10.1038","volume":"10","author":[{"given":"Davide","family":"Rovina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"La Vecchia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Cortesi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Fontana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Pesant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Maitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Tabano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Bodega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Miozzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia M.","family":"Sirchia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,19]]},"reference":[{"key":"65082_CR1","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1038\/hdy.2014.54","volume":"113","author":"CW Hanna","year":"2014","unstructured":"Hanna, C. 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