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Like effector T cells, T<jats:sub>regs<\/jats:sub> can specialize in T<jats:sub>H<\/jats:sub>1-dominated immune responses and co-express T-bet together with Foxp3. This allows for expression of CXCR3 and efficient homing to sites of T<jats:sub>H<\/jats:sub>1 responses. However, whether such functional specialization is paralleled by memory generation among T<jats:sub>regs<\/jats:sub> is unknown. In this study, we investigated the ability of polyclonal T<jats:sub>regs<\/jats:sub> to form functional memory in response to viral infection. Using adoptive transfer models to compare infection-experienced T<jats:sub>regs<\/jats:sub> generated upon acute Lymphocytic Choriomeningitis Virus (LCMV) WE and Vaccinia Virus (VV) infections with naive T<jats:sub>regs<\/jats:sub>, we observed no differences in their phenotype or their <jats:italic>in vivo<\/jats:italic> maintenance. When comparing functional properties of infection-experienced and naive T<jats:sub>regs<\/jats:sub>, we found no differences in <jats:italic>in vitro<\/jats:italic> suppressive capacity nor in their ability to limit the effector response upon homologous, systemic or local re-challenge <jats:italic>in vivo<\/jats:italic>. Our results suggest that no functional T<jats:sub>reg<\/jats:sub> memory is generated in the context of systemic LCMV or VV infection, but we cannot rule out the possibility that the generation of T<jats:sub>reg<\/jats:sub> memory may be possible in other contexts.<\/jats:p>","DOI":"10.1038\/s41598-020-65212-9","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T10:02:32Z","timestamp":1589968952000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Preceding Viral Infections Do Not Imprint Long-Term Changes in Regulatory T Cell Function"],"prefix":"10.1038","volume":"10","author":[{"given":"Felix","family":"Rost","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolas","family":"Rakebrandt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Joller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,5,20]]},"reference":[{"key":"65212_CR1","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1038\/nri.2015.1","volume":"16","author":"MD Rosenblum","year":"2016","unstructured":"Rosenblum, M. 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