{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T04:23:13Z","timestamp":1784175793871,"version":"3.55.0"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T00:00:00Z","timestamp":1589932800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T00:00:00Z","timestamp":1589932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mitogen-activated protein kinase (MAP kinase) pathways participate in regulation of several cellular processes involved in breast carcinogenesis. A number of non-coding RNAs including both microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) regulate or being regulated by MAPKs. We performed an<jats:italic>in-silico<\/jats:italic>method for identification of MAPKs with high number of interactions with miRNAs and lncRNAs. Bioinformatics approaches revealed that MAPK14 ranked first among MAPKs. Subsequently, we identified miRNAs and lncRNAs that were predicted to be associated with MAPK14. Finally, we selected four lncRNAs with higher predicted scores (<jats:italic>NORAD<\/jats:italic>,<jats:italic>HCG11<\/jats:italic>,<jats:italic>ZNRD1ASP<\/jats:italic>and<jats:italic>TTN-AS1<\/jats:italic>) and assessed their expression in 80 breast cancer tissues and their adjacent non-cancerous tissues (ANCTs). Expressions of<jats:italic>HCG11<\/jats:italic>and<jats:italic>ZNRD1ASP<\/jats:italic>were lower in tumoral tissues compared with ANCTs (P values &lt; 0.0001). However, expression levels of<jats:italic>MAPK14<\/jats:italic>and<jats:italic>NORAD<\/jats:italic>were not significantly different between breast cancer tissues and ANCTs. A significant association was detected between expression of<jats:italic>HCG11<\/jats:italic>and estrogen receptor (ER) status in a way that tumors with up-regulation of this lncRNA were mostly ER negative (P value = 0.04). Expressions of<jats:italic>ZNRD1ASP<\/jats:italic>and<jats:italic>HCG11<\/jats:italic>were associated with menopause age and breast feeding duration respectively (P values = 0.02 and 0.04 respectively). There was a trend towards association between<jats:italic>ZNRD1ASP<\/jats:italic>expression and patients\u2019 age of cancer diagnosis. Finally, we detected a trend toward association between expression of<jats:italic>NORAD<\/jats:italic>and history of hormone replacement therapy (P value = 0.06). Expression of<jats:italic>MAPK14<\/jats:italic>was significantly higher in grade 1 tumors compared with grade 2 tumors (P value = 0.02). Consequently, the current study provides evidences for association between lncRNA expressions and reproductive factors or tumor features.<\/jats:p>","DOI":"10.1038\/s41598-020-65421-2","type":"journal-article","created":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T10:03:13Z","timestamp":1589968993000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["In silico identification of MAPK14-related lncRNAs and assessment of their expression in breast cancer samples"],"prefix":"10.1038","volume":"10","author":[{"given":"Sepideh","family":"Dashti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Taherian-Esfahani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vahid","family":"Kholghi-Oskooei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rezvan","family":"Noroozi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharam","family":"Arsang-Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soudeh","family":"Ghafouri-Fard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Taheri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,20]]},"reference":[{"key":"65421_CR1","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/S0960-0760(01)00189-3","volume":"80","author":"RJ Santen","year":"2002","unstructured":"Santen, R. J. et al. The role of mitogen-activated protein (MAP) kinase in breast cancer. The Journal of steroid biochemistry and molecular biology 80, 239\u2013256 (2002).","journal-title":"The Journal of steroid biochemistry and molecular biology"},{"key":"65421_CR2","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1042\/ETLS20170142","volume":"1","author":"D Bedognetti","year":"2017","unstructured":"Bedognetti, D., Roelands, J., Decock, J., Wang, E. & Hendrickx, W. The MAPK hypothesis: immune-regulatory effects of MAPK-pathway genetic dysregulations and implications for breast cancer immunotherapy. Emerging Topics in Life Sciences 1, 429\u2013445 (2017).","journal-title":"Emerging Topics in Life Sciences"},{"key":"65421_CR3","doi-asserted-by":"publisher","first-page":"95","DOI":"10.14735\/amko201895","volume":"31","author":"B Tasharrofi","year":"2018","unstructured":"Tasharrofi, B. & Ghafouri-Fard, S. Long Non-coding RNAs as Regulators of the Mitogen-activated Protein Kinase (MAPK) Pathway in Cancer. Klinicka onkologie: casopis Ceske a Slovenske onkologicke spolecnosti 31, 95\u2013102, https:\/\/doi.org\/10.14735\/amko201895 (2018).","journal-title":"Klinicka onkologie: casopis Ceske a Slovenske onkologicke spolecnosti"},{"key":"65421_CR4","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13661","author":"D Li","year":"2018","unstructured":"Li, D. et al. Linc00483 as ceRNA regulates proliferation and apoptosis through activating MAPKs in gastric cancer. Journal of cellular and molecular medicine https:\/\/doi.org\/10.1111\/jcmm.13661 (2018).","journal-title":"Journal of cellular and molecular medicine"},{"key":"65421_CR5","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4255-3","volume":"18","author":"Y Liu","year":"2018","unstructured":"Liu, Y., Du, Y., Hu, X., Zhao, L. & Xia, W. Up-regulation of ceRNA TINCR by SP1 contributes to tumorigenesis in breast cancer. 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