{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:07:13Z","timestamp":1790647633428,"version":"4.1.0"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T00:00:00Z","timestamp":1590451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Symptoms of <jats:italic>Staphylococcus lugdunensis<\/jats:italic> infection are often similar to those of <jats:italic>Staphylococcus aureus<\/jats:italic> infection, including skin and soft-tissue lesions, bacteremia and infective endocarditis. Despite the severity of these infections, <jats:italic>S. lugdunensis<\/jats:italic> is regarded as a less important pathogen than drug-resistant <jats:italic>S. aureus<\/jats:italic>. To investigate its ability to cause infectious diseases, a methicillin-resistant <jats:italic>S. lugdunensis<\/jats:italic> (MRSL) strain JICS135 was isolated from a patient with bacteremia and subjected to whole genome sequencing. Similar to most strains of methicillin-resistant <jats:italic>S. aureus<\/jats:italic> (MRSA), this MRSL strain possessed the staphylococcal cassette chromosome <jats:italic>mec<\/jats:italic> (SCC<jats:italic>mec<\/jats:italic>) located close to the origin of replication. However, the SCC<jats:italic>mec<\/jats:italic> in this MRSL strain, with three <jats:italic>ccr<\/jats:italic> complexes, was structurally unique and currently untypable. Moreover, the SCC<jats:italic>mec<\/jats:italic> of this MRSL strain was found to carry two genes encoding microbial surface components recognizing adhesive matrix molecules (MSCRAMM)-like proteins accompanied by glycosyl transferases, one of which may have been derived from <jats:italic>S. aureus<\/jats:italic> and the other from <jats:italic>S. epidermidis<\/jats:italic>, indicating that this MRSL evolved to carry virulence factors from other staphylococci. The emergence of this strain, the first MRSL strain whose genome has been sequenced completely, may be of public concern.<\/jats:p>","DOI":"10.1038\/s41598-020-65632-7","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T10:02:38Z","timestamp":1590487358000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Complete genome sequence of a methicillin-resistant Staphylococcus lugdunensis strain and characteristics of its staphylococcal cassette chromosome mec"],"prefix":"10.1038","volume":"10","author":[{"given":"Rie","family":"Shibuya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Uehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Baba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniko","family":"Teruya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhito","family":"Satou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruo","family":"Kirikae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichi","family":"Hiramatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,26]]},"reference":[{"key":"65632_CR1","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1099\/00207713-38-2-168","volume":"38","author":"J Freney","year":"1988","unstructured":"Freney, J. et al. Staphylococcus lugdunensis sp. nov. and Staphylococcus schleiferi sp. nov., Two Species from Human Clinical Specimens. International Journal of Systematic and Evolutionary Microbiology 38, 168\u2013172, https:\/\/doi.org\/10.1099\/00207713-38-2-168 (1988).","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"65632_CR2","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1128\/CMR.00036-07","volume":"21","author":"KL Frank","year":"2008","unstructured":"Frank, K. L., Del Pozo, J. L. & Patel, R. From clinical microbiology to infection pathogenesis: how daring to be different works for Staphylococcus lugdunensis. Clin Microbiol Rev 21, 111\u2013133, https:\/\/doi.org\/10.1128\/CMR.00036-07 (2008).","journal-title":"Clin Microbiol Rev"},{"key":"65632_CR3","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1007\/s15010-008-7287-9","volume":"36","author":"AS Zinkernagel","year":"2008","unstructured":"Zinkernagel, A. S. et al. Significance of Staphylococcus lugdunensis bacteremia: report of 28 cases and review of the literature. Infection 36, 314\u2013321, https:\/\/doi.org\/10.1007\/s15010-008-7287-9 (2008).","journal-title":"Infection"},{"key":"65632_CR4","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1128\/jcm.41.1.519-520.2003","volume":"41","author":"WSN Tee","year":"2003","unstructured":"Tee, W. S. N., Soh, S. Y., Lin, R. & Loo, L. H. Staphylococcus lugdunensis Carrying the mecA Gene Causes Catheter-Associated Bloodstream Infection in Premature Neonate. Journal of Clinical Microbiology 41, 519\u2013520, https:\/\/doi.org\/10.1128\/jcm.41.1.519-520.2003 (2003).","journal-title":"Journal of Clinical Microbiology"},{"key":"65632_CR5","first-page":"99","volume":"52","author":"M Tibebu","year":"2014","unstructured":"Tibebu, M. Severe hospital acquired pneumonia and septicemia due TO methicillin resistant Staphylococcus lugdunensis in a newborn in Northwestern Ethiopia. Ethiopian medical journal 52, 99\u2013101 (2014).","journal-title":"Ethiopian medical journal"},{"key":"65632_CR6","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.diagmicrobio.2014.10.004","volume":"81","author":"PL Ho","year":"2015","unstructured":"Ho, P. L. et al. Carriage niches and molecular epidemiology of Staphylococcus lugdunensis and methicillin-resistant S. lugdunensis among patients undergoing long-term renal replacement therapy. Diagn Microbiol Infect Dis 81, 141\u2013144, https:\/\/doi.org\/10.1016\/j.diagmicrobio.2014.10.004 (2015).","journal-title":"Diagn Microbiol Infect Dis"},{"key":"65632_CR7","doi-asserted-by":"publisher","DOI":"10.1186\/1752-1947-2-267","volume":"2","author":"T Spanu","year":"2008","unstructured":"Spanu, T. et al. Ventriculitis due to Staphylococcus lugdunensis: two case reports. J Med Case Rep 2, 267, https:\/\/doi.org\/10.1186\/1752-1947-2-267 (2008).","journal-title":"J Med Case Rep"},{"key":"65632_CR8","doi-asserted-by":"publisher","first-page":"2393","DOI":"10.1128\/JCM.00740-08","volume":"46","author":"TY Tan","year":"2008","unstructured":"Tan, T. Y., Ng, S. Y. & He, J. Microbiological characteristics, presumptive identification, and antibiotic susceptibilities of Staphylococcus lugdunensis. J Clin Microbiol 46, 2393\u20132395, https:\/\/doi.org\/10.1128\/JCM.00740-08 (2008).","journal-title":"J Clin Microbiol"},{"key":"65632_CR9","doi-asserted-by":"publisher","first-page":"3015","DOI":"10.1128\/JCM.01138-11","volume":"49","author":"AB Wu","year":"2011","unstructured":"Wu, A. B. et al. Clinical and microbiological characteristics of community-acquired Staphylococcus lugdunensis infections in Southern Taiwan. J Clin Microbiol 49, 3015\u20133018, https:\/\/doi.org\/10.1128\/JCM.01138-11 (2011).","journal-title":"J Clin Microbiol"},{"key":"65632_CR10","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/j.jmii.2013.11.006","volume":"48","author":"SP Tseng","year":"2015","unstructured":"Tseng, S. P. et al. Genotypes and phenotypes of Staphylococcus lugdunensis isolates recovered from bacteremia. J Microbiol Immunol Infect 48, 397\u2013405, https:\/\/doi.org\/10.1016\/j.jmii.2013.11.006 (2015).","journal-title":"J Microbiol Immunol Infect"},{"key":"65632_CR11","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1016\/j.jmii.2013.12.010","volume":"48","author":"JF Lin","year":"2015","unstructured":"Lin, J. F. et al. Clinical experience and microbiologic characteristics of invasive Staphylococcus lugdunensis infection in a tertiary center in northern Taiwan. J Microbiol Immunol Infect 48, 406\u2013412, https:\/\/doi.org\/10.1016\/j.jmii.2013.12.010 (2015).","journal-title":"J Microbiol Immunol Infect"},{"issue":"6","key":"65632_CR12","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1016\/j.jmii.2014.11.018","volume":"49","author":"Ting-Yu Yen","year":"2016","unstructured":"Yen, T. Y. et al. Emergence of oxacillin-resistant Staphylococcus lugdunensis carrying staphylococcal cassette chromosome mec type V in central Taiwan. J Microbiol Immunol Infect 49, 885\u2013891, https:\/\/doi.org\/10.1016\/j.jmii.2014.11.018 (2016).","journal-title":"Journal of Microbiology, Immunology and Infection"},{"key":"65632_CR13","doi-asserted-by":"publisher","first-page":"5406","DOI":"10.4081\/idr.2014.5406","volume":"6","author":"S Bhumbra","year":"2014","unstructured":"Bhumbra, S., Mahboubi, M. & Blackwood, R. A. Staphylococcus lugdunensis: Novel Organism causing Cochlear Implant Infection. Infect Dis Rep 6, 5406, https:\/\/doi.org\/10.4081\/idr.2014.5406 (2014).","journal-title":"Infect Dis Rep"},{"key":"65632_CR14","doi-asserted-by":"publisher","first-page":"706","DOI":"10.4081\/cp.2015.706","volume":"5","author":"M Mehmood","year":"2015","unstructured":"Mehmood, M. & Khasawneh, F. A. Staphylococcus lugdunensis gluteal abscess in a patient with end stage renal disease on hemodialysis. Clin Pract 5, 706, https:\/\/doi.org\/10.4081\/cp.2015.706 (2015).","journal-title":"Clin Pract"},{"key":"65632_CR15","doi-asserted-by":"publisher","first-page":"405","DOI":"10.7883\/yoken.JJID.2016.130","volume":"70","author":"Y Ainoda","year":"2017","unstructured":"Ainoda, Y. et al. Multicenter Study of the Clinical Presentation of Staphylococcus lugdunensis Bacteremia in Japan. Jpn J Infect Dis 70, 405\u2013407, https:\/\/doi.org\/10.7883\/yoken.JJID.2016.130 (2017).","journal-title":"Jpn J Infect Dis"},{"key":"65632_CR16","doi-asserted-by":"publisher","first-page":"736259","DOI":"10.1155\/2014\/736259","volume":"2014","author":"AH Al-Charrakh","year":"2014","unstructured":"Al-Charrakh, A. H. & Obayes, M. H. First record of isolation and characterization of methicillin resistant Staphylococcus lugdunensis from clinical samples in Iraq. Biomed Res Int 2014, 736259, https:\/\/doi.org\/10.1155\/2014\/736259 (2014).","journal-title":"Biomed Res Int"},{"key":"65632_CR17","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1099\/jmm.0.016733-0","volume":"59","author":"F Szabados","year":"2010","unstructured":"Szabados, F., Woloszyn, J., Richter, C., Kaase, M. & Gatermann, S. Identification of molecularly defined Staphylococcus aureus strains using matrix-assisted laser desorption\/ionization time of flight mass spectrometry and the Biotyper 2.0 database. J Med Microbiol 59, 787\u2013790, https:\/\/doi.org\/10.1099\/jmm.0.016733-0 (2010).","journal-title":"J Med Microbiol"},{"key":"65632_CR18","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1099\/ijs.0.64483-0","volume":"57","author":"J Goris","year":"2007","unstructured":"Goris, J. et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57, 81\u201391, https:\/\/doi.org\/10.1099\/ijs.0.64483-0 (2007).","journal-title":"Int J Syst Evol Microbiol"},{"key":"65632_CR19","doi-asserted-by":"publisher","first-page":"19126","DOI":"10.1073\/pnas.0906412106","volume":"106","author":"M Richter","year":"2009","unstructured":"Richter, M. & Rossello-Mora, R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106, 19126\u201319131, https:\/\/doi.org\/10.1073\/pnas.0906412106 (2009).","journal-title":"Proc Natl Acad Sci USA"},{"key":"65632_CR20","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1007\/s10482-017-0844-4","volume":"110","author":"SH Yoon","year":"2017","unstructured":"Yoon, S. H., Ha, S. M., Lim, J., Kwon, S. & Chun, J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 110, 1281\u20131286, https:\/\/doi.org\/10.1007\/s10482-017-0844-4 (2017).","journal-title":"Antonie Van Leeuwenhoek"},{"issue":"6","key":"65632_CR21","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1038\/nmeth.2474","volume":"10","author":"Chen-Shan Chin","year":"2013","unstructured":"Chin, C.-S. et al. Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nature Methods 10, 563, https:\/\/doi.org\/10.1038\/nmeth.2474, https:\/\/www.nature.com\/articles\/nmeth.2474#supplementary-information (2013).","journal-title":"Nature Methods"},{"key":"65632_CR22","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-64","volume":"8","author":"DD Sommer","year":"2007","unstructured":"Sommer, D. D., Delcher, A. L., Salzberg, S. L. & Pop, M. Minimus: a fast, lightweight genome assembler. BMC Bioinformatics 8, 64, https:\/\/doi.org\/10.1186\/1471-2105-8-64 (2007).","journal-title":"BMC Bioinformatics"},{"key":"65632_CR23","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75","volume":"9","author":"RK Aziz","year":"2008","unstructured":"Aziz, R. K. et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9, 75, https:\/\/doi.org\/10.1186\/1471-2164-9-75 (2008).","journal-title":"BMC Genomics"},{"key":"65632_CR24","doi-asserted-by":"publisher","first-page":"1355","DOI":"10.1128\/JCM.06094-11","volume":"50","author":"MV Larsen","year":"2012","unstructured":"Larsen, M. V. et al. Multilocus sequence typing of total-genome-sequenced bacteria. J Clin Microbiol 50, 1355\u20131361, https:\/\/doi.org\/10.1128\/JCM.06094-11 (2012).","journal-title":"J Clin Microbiol"},{"key":"65632_CR25","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1128\/JCM.03617-13","volume":"52","author":"KG Joensen","year":"2014","unstructured":"Joensen, K. G. et al. Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli. J Clin Microbiol 52, 1501\u20131510, https:\/\/doi.org\/10.1128\/JCM.03617-13 (2014).","journal-title":"J Clin Microbiol"},{"key":"65632_CR26","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421","volume":"10","author":"C Camacho","year":"2009","unstructured":"Camacho, C. et al. BLAST+: architecture and applications. BMC Bioinformatics 10, 421, https:\/\/doi.org\/10.1186\/1471-2105-10-421 (2009).","journal-title":"BMC Bioinformatics"},{"key":"65632_CR27","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2336-6","volume":"19","author":"P Clausen","year":"2018","unstructured":"Clausen, P., Aarestrup, F. M. & Lund, O. Rapid and precise alignment of raw reads against redundant databases with KMA. BMC Bioinformatics 19, 307, https:\/\/doi.org\/10.1186\/s12859-018-2336-6 (2018).","journal-title":"BMC Bioinformatics"},{"key":"65632_CR28","doi-asserted-by":"publisher","first-page":"e104984","DOI":"10.1371\/journal.pone.0104984","volume":"9","author":"RS Kaas","year":"2014","unstructured":"Kaas, R. S., Leekitcharoenphon, P., Aarestrup, F. M. & Lund, O. Solving the problem of comparing whole bacterial genomes across different sequencing platforms. Plos One 9, e104984, https:\/\/doi.org\/10.1371\/journal.pone.0104984 (2014).","journal-title":"Plos One"},{"key":"65632_CR29","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1007\/bf01734359","volume":"17","author":"J Felsenstein","year":"1981","unstructured":"Felsenstein, J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368\u2013376, https:\/\/doi.org\/10.1007\/bf01734359 (1981).","journal-title":"J Mol Evol"},{"key":"65632_CR30","doi-asserted-by":"publisher","first-page":"1471","DOI":"10.1128\/JB.01627-09","volume":"192","author":"H Tse","year":"2010","unstructured":"Tse, H. et al. Complete genome sequence of Staphylococcus lugdunensis strain HKU09-01. J Bacteriol 192, 1471\u20131472, https:\/\/doi.org\/10.1128\/JB.01627-09 (2010).","journal-title":"J Bacteriol"},{"key":"65632_CR31","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1111\/j.1574-6968.2011.02339.x","volume":"322","author":"S Heilbronner","year":"2011","unstructured":"Heilbronner, S. et al. Genome sequence of Staphylococcus lugdunensis N920143 allows identification of putative colonization and virulence factors. FEMS Microbiol Lett 322, 60\u201367, https:\/\/doi.org\/10.1111\/j.1574-6968.2011.02339.x (2011).","journal-title":"FEMS Microbiol Lett"},{"key":"65632_CR32","doi-asserted-by":"publisher","first-page":"13272","DOI":"10.1073\/pnas.0502950102","volume":"102","author":"M Kuroda","year":"2005","unstructured":"Kuroda, M. et al. Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection. Proc Natl Acad Sci U S A 102, 13272\u201313277, https:\/\/doi.org\/10.1073\/pnas.0502950102 (2005).","journal-title":"Proc Natl Acad Sci U S A"},{"key":"65632_CR33","doi-asserted-by":"publisher","unstructured":"Shiroma, A. et al. First Complete Genome Sequences of Staphylococcus aureus subsp. aureus Rosenbach 1884 (DSM 20231T), Determined by PacBio Single-Molecule Real-Time Technology. Genome Announc 3, https:\/\/doi.org\/10.1128\/genomeA.00800-15 (2015).","DOI":"10.1128\/genomeA.00800-15"},{"key":"65632_CR34","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1016\/s0140-6736(00)04403-2","volume":"357","author":"M Kuroda","year":"2001","unstructured":"Kuroda, M. et al. Whole genome sequencing of meticillin-resistant Staphylococcus aureus. Lancet (London, England) 357, 1225\u20131240, https:\/\/doi.org\/10.1016\/s0140-6736(00)04403-2 (2001).","journal-title":"Lancet (London, England)"},{"key":"65632_CR35","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1128\/aac.44.6.1549-1555.2000","volume":"44","author":"Y Katayama","year":"2000","unstructured":"Katayama, Y., Ito, T. & Hiramatsu, K. A new class of genetic element, staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob Agents Chemother 44, 1549\u20131555, https:\/\/doi.org\/10.1128\/aac.44.6.1549-1555.2000 (2000).","journal-title":"Antimicrob Agents Chemother"},{"key":"65632_CR36","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1128\/AAC.00165-06","volume":"51","author":"Y Kondo","year":"2007","unstructured":"Kondo, Y. et al. Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother 51, 264\u2013274, https:\/\/doi.org\/10.1128\/AAC.00165-06 (2007).","journal-title":"Antimicrob Agents Chemother"},{"key":"65632_CR37","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1146\/annurev.mi.48.100194.003101","volume":"48","author":"JM Patti","year":"1994","unstructured":"Patti, J. M., Allen, B. L., McGavin, M. J. & Hook, M. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol 48, 585\u2013617, https:\/\/doi.org\/10.1146\/annurev.mi.48.100194.003101 (1994).","journal-title":"Annu Rev Microbiol"},{"key":"65632_CR38","doi-asserted-by":"publisher","first-page":"6660","DOI":"10.1093\/emboj\/cdf619","volume":"21","author":"CC Deivanayagam","year":"2002","unstructured":"Deivanayagam, C. C. et al. A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A. EMBO J 21, 6660\u20136672 (2002).","journal-title":"EMBO J"},{"key":"65632_CR39","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1038\/nrmicro3161","volume":"12","author":"TJ Foster","year":"2014","unstructured":"Foster, T. J., Geoghegan, J. A., Ganesh, V. K. & Hook, M. Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nat Rev Microbiol 12, 49\u201362, https:\/\/doi.org\/10.1038\/nrmicro3161 (2014).","journal-title":"Nat Rev Microbiol"},{"key":"65632_CR40","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/0092-8674(92)90101-H","volume":"70","author":"O Schneewind","year":"1992","unstructured":"Schneewind, O., Model, P. & Fischetti, V. A. Sorting of protein A to the staphylococcal cell wall. Cell 70, 267\u2013281 (1992).","journal-title":"Cell"},{"key":"65632_CR41","doi-asserted-by":"publisher","first-page":"4803","DOI":"10.1002\/j.1460-2075.1993.tb06169.x","volume":"12","author":"O Schneewind","year":"1993","unstructured":"Schneewind, O., Mihaylova-Petkov, D. & Model, P. Cell wall sorting signals in surface proteins of gram-positive bacteria. EMBO J 12, 4803\u20134811 (1993).","journal-title":"EMBO J"},{"key":"65632_CR42","doi-asserted-by":"publisher","first-page":"1081","DOI":"10.1046\/j.1365-2958.2002.02949.x","volume":"44","author":"BA Bensing","year":"2002","unstructured":"Bensing, B. A. & Sullam, P. M. An accessory sec locus of Streptococcus gordonii is required for export of the surface protein GspB and for normal levels of binding to human platelets. Mol Microbiol 44, 1081\u20131094 (2002).","journal-title":"Mol Microbiol"},{"key":"65632_CR43","doi-asserted-by":"publisher","first-page":"6188","DOI":"10.1128\/JB.00300-08","volume":"190","author":"IR Siboo","year":"2008","unstructured":"Siboo, I. R., Chaffin, D. O., Rubens, C. E. & Sullam, P. M. Characterization of the accessory Sec system of Staphylococcus aureus. J Bacteriol 190, 6188\u20136196, https:\/\/doi.org\/10.1128\/JB.00300-08 (2008).","journal-title":"J Bacteriol"},{"key":"65632_CR44","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1099\/jmm.0.001013","volume":"68","author":"MC-J Liu","year":"2019","unstructured":"Liu, M. C.-J. et al. Structures of SCC mec elements in methicillin-resistant Staphylococcus lugdunensis are closely related to those harboured by community-associated methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology 68, 1367\u20131372, \u00a0https:\/\/doi.org\/10.1099\/jmm.0.001013 (2019).","journal-title":"Journal of Medical Microbiology"},{"key":"65632_CR45","doi-asserted-by":"publisher","first-page":"3258","DOI":"10.1093\/jac\/dkx291","volume":"72","author":"SC Chang","year":"2017","unstructured":"Chang, S. C. et al. Characterization of two novel variants of staphylococcal cassette chromosome mec elements in oxacillin-resistant Staphylococcus lugdunensis. J Antimicrob Chemother 72, 3258\u20133262, https:\/\/doi.org\/10.1093\/jac\/dkx291 (2017).","journal-title":"J Antimicrob Chemother"},{"key":"65632_CR46","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.meegid.2018.03.012","volume":"61","author":"AM O\u2019Connor","year":"2018","unstructured":"O\u2019Connor, A. M., McManus, B. A. & Coleman, D. C. First description of novel arginine catabolic mobile elements (ACMEs) types IV and V harboring a kdp operon in Staphylococcus epidermidis characterized by whole genome sequencing. Infect Genet Evol 61, 60\u201366, https:\/\/doi.org\/10.1016\/j.meegid.2018.03.012 (2018).","journal-title":"Infect Genet Evol"},{"key":"65632_CR47","doi-asserted-by":"publisher","first-page":"2426","DOI":"10.1128\/JB.187.7.2426-2438.2005","volume":"187","author":"SR Gill","year":"2005","unstructured":"Gill, S. R. et al. Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. J Bacteriol 187, 2426\u20132438, https:\/\/doi.org\/10.1128\/JB.187.7.2426-2438.2005 (2005).","journal-title":"J Bacteriol"},{"key":"65632_CR48","doi-asserted-by":"publisher","first-page":"1819","DOI":"10.1016\/s0140-6736(02)08713-5","volume":"359","author":"T Baba","year":"2002","unstructured":"Baba, T. et al. Genome and virulence determinants of high virulence community-acquired MRSA. The Lancet 359, 1819\u20131827, https:\/\/doi.org\/10.1016\/s0140-6736(02)08713-5 (2002).","journal-title":"The Lancet"},{"key":"65632_CR49","doi-asserted-by":"publisher","first-page":"6411","DOI":"10.1128\/AAC.00948-16","volume":"60","author":"PL Ho","year":"2016","unstructured":"Ho, P. L. et al. Emergence of ileS2-Carrying, Multidrug-Resistant Plasmids in Staphylococcus lugdunensis. Antimicrob Agents Chemother 60, 6411\u20136414, https:\/\/doi.org\/10.1128\/AAC.00948-16 (2016).","journal-title":"Antimicrob Agents Chemother"},{"key":"65632_CR50","doi-asserted-by":"publisher","first-page":"7292","DOI":"10.1128\/JB.187.21.7292-7308.2005","volume":"187","author":"F Takeuchi","year":"2005","unstructured":"Takeuchi, F. et al. Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species. J Bacteriol 187, 7292\u20137308, https:\/\/doi.org\/10.1128\/JB.187.21.7292-7308.2005 (2005).","journal-title":"J Bacteriol"},{"key":"65632_CR51","doi-asserted-by":"publisher","first-page":"3003","DOI":"10.1128\/JCM.00988-12","volume":"50","author":"B Chassain","year":"2012","unstructured":"Chassain, B. et al. Multilocus sequence typing analysis of Staphylococcus lugdunensis implies a clonal population structure. J Clin Microbiol 50, 3003\u20133009, https:\/\/doi.org\/10.1128\/JCM.00988-12 (2012).","journal-title":"J Clin Microbiol"},{"key":"65632_CR52","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.ijid.2015.05.022","volume":"36","author":"CW Cheng","year":"2015","unstructured":"Cheng, C. W. et al. Persistence of a major endemic clone of oxacillin-resistant Staphylococcus lugdunensis sequence type 6 at a tertiary medical centre in northern Taiwan. Int J Infect Dis 36, 72\u201377, https:\/\/doi.org\/10.1016\/j.ijid.2015.05.022 (2015).","journal-title":"Int J Infect Dis"},{"key":"65632_CR53","doi-asserted-by":"publisher","first-page":"2051","DOI":"10.1128\/JCM.00847-16","volume":"54","author":"CF Yeh","year":"2016","unstructured":"Yeh, C. F. et al. Clinical Features, Outcomes, and Molecular Characteristics of Community- and Health Care-Associated Staphylococcus lugdunensis Infections. J Clin Microbiol 54, 2051\u20132057, https:\/\/doi.org\/10.1128\/JCM.00847-16 (2016).","journal-title":"J Clin Microbiol"},{"key":"65632_CR54","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1074\/jbc.M112.385138","volume":"288","author":"S Boundy","year":"2013","unstructured":"Boundy, S. et al. Characterization of the Staphylococcus aureus rRNA methyltransferase encoded by orfX, the gene containing the staphylococcal chromosome Cassette mec (SCCmec) insertion site. J Biol Chem 288, 132\u2013140, https:\/\/doi.org\/10.1074\/jbc.M112.385138 (2013).","journal-title":"J Biol Chem"},{"key":"65632_CR55","doi-asserted-by":"publisher","first-page":"2105","DOI":"10.1093\/jac\/dkw123","volume":"71","author":"P Liu","year":"2016","unstructured":"Liu, P., Xue, H., Wu, Z., Ma, J. & Zhao, X. Effect of bla regulators on the susceptible phenotype and phenotypic conversion for oxacillin-susceptible mecA-positive staphylococcal isolates. J Antimicrob Chemother 71, 2105\u20132112, https:\/\/doi.org\/10.1093\/jac\/dkw123 (2016).","journal-title":"J Antimicrob Chemother"},{"key":"65632_CR56","doi-asserted-by":"publisher","first-page":"12312","DOI":"10.1073\/pnas.0404728101","volume":"101","author":"T Bae","year":"2004","unstructured":"Bae, T. et al. Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing. Proc Natl Acad Sci U S A 101, 12312\u201312317, https:\/\/doi.org\/10.1073\/pnas.0404728101 (2004).","journal-title":"Proc Natl Acad Sci U S A"},{"key":"65632_CR57","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11306-6","volume":"10","author":"H Sichtig","year":"2019","unstructured":"Sichtig, H. et al. FDA-ARGOS is a database with public quality-controlled reference genomes for diagnostic use and regulatory science. Nat Commun 10, 3313, https:\/\/doi.org\/10.1038\/s41467-019-11306-6 (2019).","journal-title":"Nat Commun"},{"key":"65632_CR58","doi-asserted-by":"publisher","unstructured":"Argemi, X. et al. Whole-Genome Sequencing of Seven Strains of Staphylococcus lugdunensis Allows Identification of Mobile Genetic Elements. Genome Biol Evol 9, https:\/\/doi.org\/10.1093\/gbe\/evx077 (2017).","DOI":"10.1093\/gbe\/evx077"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-65632-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-65632-7","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-65632-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T11:34:04Z","timestamp":1670326444000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-65632-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,26]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["65632"],"URL":"https:\/\/doi.org\/10.1038\/s41598-020-65632-7","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,26]]},"assertion":[{"value":"26 November 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 May 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 May 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"8682"}}