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Although improvements in diagnosis and treatment have led to a decline in mortality rates, morbidity rates have remained relatively unchanged. Bacterial resistance to antibiotics in this clinical setting further underlines the need for developing other technologies, such as phage therapy. We exploited an <jats:italic>in vitro<\/jats:italic> phage therapy model for studying bacterial neonatal meningitis based on <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E. coli<\/jats:italic>) EV36, bacteriophage (phage) K1F and human cerebral microvascular endothelial cells (hCMECs). We show that phage K1F is phagocytosed and degraded by constitutive- and PAMP-dependent LC3-assisted phagocytosis and does not induce expression of inflammatory cytokines TNF\u03b1, IL-6, IL-8 or IFN\u03b2. Additionally, we observed that phage K1F temporarily decreases the barrier resistance of hCMEC cultures, a property that influences the barrier permeability, which could facilitate the transition of immune cells across the endothelial vessel <jats:italic>in vivo<\/jats:italic>. Collectively, we demonstrate that phage K1F can infect intracellular <jats:italic>E. coli<\/jats:italic> EV36 within hCMECs without themselves eliciting an inflammatory or defensive response. This study illustrates the potential of phage therapy targeting infections such as bacterial neonatal meningitis and is an important step for the continued development of phage therapy targeting antibiotic-resistant bacterial infections generally.<\/jats:p>","DOI":"10.1038\/s41598-020-65867-4","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T10:03:14Z","timestamp":1591005794000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Bacteriophage K1F targets Escherichia coli K1 in cerebral endothelial cells and influences the barrier function"],"prefix":"10.1038","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6944-5601","authenticated-orcid":false,"given":"Christian","family":"M\u00f8ller-Olsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7277-6949","authenticated-orcid":false,"given":"Toby","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2155-7919","authenticated-orcid":false,"given":"Keith N.","family":"Leppard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7004-1324","authenticated-orcid":false,"given":"Veronica","family":"Foisor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3364-7946","authenticated-orcid":false,"given":"Corinne","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6296-8290","authenticated-orcid":false,"given":"Dimitris K.","family":"Grammatopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0386-2322","authenticated-orcid":false,"given":"Antonia P.","family":"Sagona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,1]]},"reference":[{"key":"65867_CR1","doi-asserted-by":"publisher","first-page":"1781","DOI":"10.1099\/jmm.0.059840-0","volume":"62","author":"T Barichello","year":"2013","unstructured":"Barichello, T. et al. 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