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Rapid detection of\n                    <jats:italic>Lp<\/jats:italic>\n                    in water system is essential to reduce the risk of LD outbreaks. The methods currently available require expert skills and are time intensive, thus delaying intervention.\n                    <jats:italic>In situ<\/jats:italic>\n                    detection of\n                    <jats:italic>Lp<\/jats:italic>\n                    by biosensor would allow rapid implementation of control strategies. To this end, a biorecognition element is required. Aptamers are considered promising biorecognition molecules for biosensing. Aptamers are short oligonucleotide sequence folding into a specific structure and are able to bind to specific molecules. Currently, no aptamer and thus no aptamer-based technology exists for the detection of\n                    <jats:italic>Lp<\/jats:italic>\n                    . In this study, Systemic Evolution of Ligands through EXponential enrichment (SELEX) was used to identify aptamers binding specifically to\n                    <jats:italic>Lp<\/jats:italic>\n                    . Ten rounds of positive selection and two rounds of counter-selection against two\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    species were performed. Two aptamers binding strongly to\n                    <jats:italic>Lp<\/jats:italic>\n                    were identified with\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>D<\/jats:italic>\n                    <\/jats:sub>\n                    of 116 and 135\u2009nM. Binding specificity of these two aptamers to\n                    <jats:italic>Lp<\/jats:italic>\n                    was confirmed by flow cytometry and fluorescence microscopy. Therefore, these two aptamers are promising biorecognition molecules for the detection of\n                    <jats:italic>Lp<\/jats:italic>\n                    in water systems.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-65973-3","type":"journal-article","created":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T06:03:02Z","timestamp":1591250582000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Identification of two aptamers binding to Legionella pneumophila with high affinity and specificity"],"prefix":"10.1038","volume":"10","author":[{"given":"Mariam","family":"Saad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deanna","family":"Chinerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Tabrizian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2721-7715","authenticated-orcid":false,"given":"Sebastien P.","family":"Faucher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,4]]},"reference":[{"issue":"22","key":"65973_CR1","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1056\/NEJM197712012972202","volume":"297","author":"JE McDade","year":"1977","unstructured":"McDade, J. 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