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One of the mechanisms of resistance within a biofilm is retardation of drug diffusion due to poor penetration across the matrix. To overcome this challenge, this work set to investigate novel counter ion approach with acidic amino acids, which we hypothesised will disrupt the biofilm matrix as well as simultaneously improve drug effectiveness. The anti-biofilm activity of D-Asp and D-Glu was studied on\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    biofilms. Synergistic effect of combining D-amino acids with Cip was also investigated as a strategy to overcome anti-microbial resistance in these biofilms. Interestingly at equimolar combinations, D-Asp and D-Glu were able to significantly disperse (at 20\u2009mM and 40\u2009mM) established biofilms and inhibit (at 10\u2009mM, 20\u2009mM and 40\u2009mM) new biofilm formation in the absence of an antibiotic. Moreover, our study confirmed L-amino acids also exhibit anti-biofilm activity. The synergistic effect of acidic amino acids with Cip was observed at lower concentration ranges (&lt;40\u2009mM amino acids and &lt;90.54\u2009\u00b5M, respectively), which resulted in 96.89% (inhibition) and 97.60% (dispersal) reduction in CFU with exposure to 40\u2009mM amino acids. Confocal imaging indicated that the amino acids disrupt the honeycomb-like extracellular DNA (eDNA) meshwork whilst also preventing its formation.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-66082-x","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T06:06:02Z","timestamp":1591077962000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["Evaluation of anti-biofilm activity of acidic amino acids and synergy with ciprofloxacin on Staphylococcus aureus biofilms"],"prefix":"10.1038","volume":"10","author":[{"given":"Annsar A.","family":"Warraich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afzal R.","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Perrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majad","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazel","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6971-8825","authenticated-orcid":false,"given":"Ayesha","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,2]]},"reference":[{"key":"66082_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00203-015-1148-6","volume":"198","author":"P Gupta","year":"2016","unstructured":"Gupta, P., Sarkar, S., Das, B., Bhattacharjee, S. & Tribedi, P. 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