{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:34:37Z","timestamp":1791387277295,"version":"4.3.3"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T00:00:00Z","timestamp":1592265600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T00:00:00Z","timestamp":1592265600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["1910180"],"award-info":[{"award-number":["1910180"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["1810920"],"award-info":[{"award-number":["1810920"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["1910180"],"award-info":[{"award-number":["1910180"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["1810920"],"award-info":[{"award-number":["1810920"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["1910180"],"award-info":[{"award-number":["1910180"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["1810920"],"award-info":[{"award-number":["1810920"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003645","name":"National Cancer Center","doi-asserted-by":"publisher","award":["1810920"],"award-info":[{"award-number":["1810920"]}],"id":[{"id":"10.13039\/501100003645","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The cervical microbiome is associated with cervical cancer risk, but how microbial diversity and functional profiles change in cervical cancer remains unclear. Herein, we investigated microbial-compositional and functional differences between a control group and a high-grade cervical intraepithelial neoplasia and cervical cancer (CIN2\/3-CC) group. After retrospective collection of 92 cervical swab samples, we carried out 16S rRNA amplicon sequencing on 50 and 42 samples from the control and CIN2\/3-CC groups, respectively. The EzBioCloud pipeline was applied to identify the genomic features associated with the groups using 16S rRNA data. A linear discriminant analysis effect size (LEfSe) was performed to assess the enrichment in the assigned taxonomic and functional profiles. We found a lower richness in the control group relative to the CIN2\/3-CC group; however, the \u03b2-diversity tended to be similar between the groups. The LEfSe analysis showed that a phylum\n                    <jats:italic>Sacchaaribacteria<\/jats:italic>\n                    _TM7, 11 genera, and 21 species were more abundant in the CIN2\/3-CC group and that one uncharacterized\n                    <jats:italic>Gardnerella<\/jats:italic>\n                    species was more abundant only in the control group. Further characterization of the functional pathways using EzBioCloud showed that the 4 KEGG orthologs (Phosphotransferase system [PTS] sucrose-specific IIA, IIB, IIC components and PTS cellubiose-specific IIC component) were involved in the KEGG pathway of starch and sucrose metabolism. The two pathways of folate biosynthesis and oxidative phosphorylation were more abundant in the CIN2\/3-CC group. Further confirmation of these results in larger samples can help to elucidate the potential association between the cervical microbiome and cervical cancer.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-66607-4","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T06:04:43Z","timestamp":1592287483000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Taxonomic and Functional Differences in Cervical Microbiome Associated with Cervical Cancer Development"],"prefix":"10.1038","volume":"10","author":[{"given":"Charles Nkufi","family":"Tango","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Soo","family":"Seo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minji","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Ock","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha Kyun","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5279-4162","authenticated-orcid":false,"given":"Mi Kyung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,16]]},"reference":[{"key":"66607_CR1","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1038\/modpathol.2010.85","volume":"23","author":"EY Chen","year":"2010","unstructured":"Chen, E. Y. et al. Histological \u2018progression\u2019from low (LSIL) to high (HSIL) squamous intraepithelial lesion is an uncommon event and an indication for quality assurance review. Mod. Pathol. 23, 1045\u20131051 (2010).","journal-title":"Mod. Pathol."},{"key":"66607_CR2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23389-0","volume":"8","author":"H Zhang","year":"2018","unstructured":"Zhang, H. et al. Cervical microbiome is altered in cervical intraepithelial neoplasia after loop electrosurgical excision procedure in china. Sci. Rep. 8, 4923, https:\/\/doi.org\/10.1038\/s41598-018-23389-0 (2018).","journal-title":"Sci. Rep."},{"key":"66607_CR3","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.trsl.2016.07.004","volume":"179","author":"M Kyrgiou","year":"2017","unstructured":"Kyrgiou, M., Mitra, A. & Moscicki, A. B. Does the vaginal microbiota play a role in the development of cervical cancer? Transl. Res. 179, 168\u2013182 (2017).","journal-title":"Transl. Res."},{"key":"66607_CR4","doi-asserted-by":"publisher","first-page":"1745","DOI":"10.1093\/ije\/dyx050","volume":"46","author":"SN Adebamowo","year":"2017","unstructured":"Adebamowo, S. N. et al. Cohort Profile: African Collaborative Center for Microbiome and Genomics Research\u2019s (ACCME\u2019s) Human Papillomavirus (HPV) and Cervical Cancer Study. Int. J. Epidemiol 46, 1745\u20131745j (2017).","journal-title":"Int. J. Epidemiol"},{"key":"66607_CR5","doi-asserted-by":"publisher","first-page":"1434","DOI":"10.1016\/j.clnu.2016.03.019","volume":"35","author":"SS Seo","year":"2016","unstructured":"Seo, S. S. et al. Combined effect of diet and cervical microbiome on the risk of cervical intraepithelial neoplasia. Clin. Nutr. 35, 1434\u20131441 (2016).","journal-title":"Clin. Nutr."},{"key":"66607_CR6","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1016\/j.tim.2017.11.002","volume":"26","author":"A Heintz-Buschart","year":"2018","unstructured":"Heintz-Buschart, A. & Wilmes, P. Human gut microbiome: function matters. Trends Microbiol. 26, 563\u2013574 (2018).","journal-title":"Trends Microbiol."},{"key":"66607_CR7","doi-asserted-by":"publisher","first-page":"3099","DOI":"10.1200\/JCO.2018.36.15_suppl.3099","volume":"36","author":"R Ravilla","year":"2018","unstructured":"Ravilla, R. et al. Cervical microbiome role in outcomes of therapeutic HPV vaccination for cervical intraepithelial neoplasia. Am. Soc. Clin. Onc 36, 3099\u20133099 (2018).","journal-title":"Am. Soc. Clin. Onc"},{"key":"66607_CR8","doi-asserted-by":"publisher","first-page":"12 pages","DOI":"10.1155\/2018\/2037838","volume":"Article ID 2037","author":"JE Beliz\u00e1rio","year":"2018","unstructured":"Beliz\u00e1rio, J. E., Faintuch, J. & Garay-Malpartida, M. Gut Microbiome Dysbiosis and Immunometabolism: New Frontiers for Treatment of Metabolic. Diseases. Med. Inflam Article ID 2037838, 12 pages, https:\/\/doi.org\/10.1155\/2018\/2037838 (2018).","journal-title":"Diseases. Med. Inflam"},{"key":"66607_CR9","doi-asserted-by":"publisher","first-page":"E309","DOI":"10.3390\/cancers11030309","volume":"11","author":"M Kwon","year":"2019","unstructured":"Kwon, M., Seo, S. S., Kim, M. K., Lee, D. O. & Lim, M. C. Compositional and Functional Differences between Microbiota and Cervical Carcinogenesis as Identified by Shotgun Metagenomic Sequencing. Cancers (Basel) 11, E309, https:\/\/doi.org\/10.3390\/cancers11030309 (2019).","journal-title":"Cancers (Basel)"},{"key":"66607_CR10","doi-asserted-by":"publisher","first-page":"E511","DOI":"10.3390\/cancers11040511","volume":"11","author":"I Khan","year":"2019","unstructured":"Khan, I. et al. LC\/MS-Based Polar Metabolite Profiling Identified Unique Biomarker Signatures for Cervical Cancer and Cervical Intraepithelial Neoplasia Using Global and Targeted Metabolomics. Cancers (Basel) 11, E511, https:\/\/doi.org\/10.3390\/cancers11040511 (2019).","journal-title":"Cancers (Basel)"},{"key":"66607_CR11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-33750-y","volume":"8","author":"S Arokiyaraj","year":"2018","unstructured":"Arokiyaraj, S., Seo, S. S., Kwon, M., Lee, J. K. & Kim, M. K. Association of cervical microbial community with persistence, clearance and negativity of Human Papillomavirus in Korean women: a longitudinal study. Sci. Rep. 8, 15479, https:\/\/doi.org\/10.1038\/s41598-018-33750-y (2018).","journal-title":"Sci. Rep."},{"key":"66607_CR12","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.cca.2015.01.003","volume":"451","author":"V D\u2019Argenio","year":"2015","unstructured":"D\u2019Argenio, V. & Salvatore The role of the gut microbiome in the healthy adult status. Clin. Chim. Acta 451, 97\u2013102 (2015).","journal-title":"Clin. Chim. Acta"},{"key":"66607_CR13","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1016\/j.bbrc.2015.12.083","volume":"469","author":"R Ranjan","year":"2016","unstructured":"Ranjan, R., Rani, A., Metwally, A., McGee, H. S. & Perkins, D. L. Analysis of the microbiome: Advantages of whole genome shotgun versus 16S amplicon sequencing. Biochem. Biophys. Res. Commun. 469, 967\u2013977 (2016).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"66607_CR14","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.jbiotec.2017.08.012","volume":"261","author":"S J\u00fcnemann","year":"2017","unstructured":"J\u00fcnemann, S. et al. Bioinformatics for NGS-based metagenomics and the application to biogas research. J. Biotechnol. 261, 10\u201323 (2017).","journal-title":"J. Biotechnol."},{"key":"66607_CR15","first-page":"77","volume":"5","author":"B Ji","year":"2015","unstructured":"Ji, B. & Nielsen, J. New insight into the gut microbiome through metagenomics. Adv. Genomics Genet 5, 77\u201391 (2015).","journal-title":"Adv. Genomics Genet"},{"key":"66607_CR16","doi-asserted-by":"publisher","first-page":"217","DOI":"10.3164\/jcbn.17-44","volume":"61","author":"R Inoue","year":"2017","unstructured":"Inoue, R. et al. Prediction of functional profiles of gut microbiota from 16S rRNA metagenomic data provides a more robust evaluation of gut dysbiosis occurring in Japanese type 2 diabetic patients. J. Clin. Biochem. Nutr. 61, 217\u2013221 (2017).","journal-title":"J. Clin. Biochem. Nutr."},{"key":"66607_CR17","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1136\/gutjnl-2017-314205","volume":"67","author":"RM Ferreira","year":"2018","unstructured":"Ferreira, R. M. et al. Gastric microbial community profiling reveals a dysbiotic cancer-associated microbiota. Gut. 67, 226\u2013236 (2018).","journal-title":"Gut."},{"key":"66607_CR18","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1038\/nbt.2676","volume":"31","author":"MG Langille","year":"2013","unstructured":"Langille, M. G. et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat. Biotechnol. 31, 814\u2013821 (2013).","journal-title":"Nat. Biotechnol."},{"key":"66607_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.oraloncology.2017.12.005","volume":"77","author":"SF Yang","year":"2018","unstructured":"Yang, S. F. et al. Compositional and functional variations of oral microbiota associated with the mutational changes in oral cancer. Oral. Oncol. 77, 1\u20138 (2018).","journal-title":"Oral. Oncol."},{"key":"66607_CR20","doi-asserted-by":"publisher","first-page":"e02785","DOI":"10.1128\/mBio.02785-18","volume":"10","author":"C Klein","year":"2019","unstructured":"Klein, C. et al. Relationship between the Cervical Microbiome, HIV Status, and Precancerous Lesions. mBio. 10, e02785\u201318, https:\/\/doi.org\/10.1128\/mBio.02785-18 (2019).","journal-title":"mBio."},{"key":"66607_CR21","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1158\/1940-6207.CAPR-15-0350","volume":"9","author":"CJ Piyathilake","year":"2016","unstructured":"Piyathilake, C. J. Cervical microbiota associated with higher grade cervical intraepithelial neoplasia in women infected with high-risk human papillomaviruses. Canc. Prev. Res 9, 357\u2013366 (2016).","journal-title":"Canc. Prev. Res"},{"key":"66607_CR22","doi-asserted-by":"publisher","DOI":"10.1038\/srep16865","volume":"5","author":"A Mitra","year":"2015","unstructured":"Mitra, A. et al. Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity. Sci. Rep. 5, 16865, https:\/\/doi.org\/10.1038\/srep16865 (2015).","journal-title":"Sci. Rep."},{"key":"66607_CR23","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-018-1501-6","volume":"19","author":"KL Greathouse","year":"2018","unstructured":"Greathouse, K. L. et al. Interaction between the microbiome and TP53 in human lung cancer. Genome Biol. 19, 123, https:\/\/doi.org\/10.1186\/s13059-018-1501-6 (2018).","journal-title":"Genome Biol."},{"key":"66607_CR24","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1158\/1940-6207.CAPR-18-0233","volume":"12","author":"W Ritu","year":"2019","unstructured":"Ritu, W. et al. Evaluation of the associations between cervical microbiota and HPV infection, clearance, and persistence in cytologically nomal women. Cancer Prev. Res (Phila) 12, 43\u201356 (2019).","journal-title":"Cancer Prev. Res (Phila)"},{"key":"66607_CR25","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.cmet.2016.08.009","volume":"25","author":"GS Ducker","year":"2017","unstructured":"Ducker, G. S. & Rabinowitz, J. D. One-carbon metabolism in health and disease. Cell Metab. 25, 27\u201342 (2017).","journal-title":"Cell Metab."},{"key":"66607_CR26","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/j.chom.2013.07.007","volume":"14","author":"AD Kostic","year":"2013","unstructured":"Kostic, A. D. et al. Fusobacterium nucleatum potentiates intestinal tumorigenesis and modulates the tumor-immune microenvironment. Cell host microbe 14, 207\u2013215 (2013).","journal-title":"Cell host microbe"},{"key":"66607_CR27","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1016\/j.eururo.2018.06.033","volume":"74","author":"MA Liss","year":"2018","unstructured":"Liss, M. A. et al. Metabolic biosynthesis pathways identified from fecal Microbiome associated with prostate cancer. Eur. Urol. 74, 575\u2013582 (2018).","journal-title":"Eur. Urol."},{"key":"66607_CR28","first-page":"1963","volume":"15","author":"Q Li","year":"2018","unstructured":"Li, Q. et al. Folate deficiency and aberrant DNA methylation and expression of FHIT gene were associated with cervical pathogenesis. Oncol. Lett 15, 1963\u20131972 (2018).","journal-title":"Oncol. Lett"},{"key":"66607_CR29","first-page":"1409","volume":"11","author":"ID Goldman","year":"2010","unstructured":"Goldman, I. D., Chattopadhyay, S., Zhao, R. & Moran, R. The antifolates: evolution, new agents in the clinic, and how targeting delivery via specific membrane transporters is driving the development of a next generation of folate analogs. Curr. Opin. Investig. Drugs. 11, 1409\u20131423 (2010).","journal-title":"Curr. Opin. Investig. Drugs."},{"key":"66607_CR30","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1016\/j.beha.2009.09.004","volume":"22","author":"JR Bertino","year":"2009","unstructured":"Bertino, J. R. Cancer research: from folate antagonism to molecular targets. Best Pract. Res. Clin. Haematol. 22, 577\u2013582 (2009).","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"66607_CR31","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1126\/science.1160809","volume":"324","author":"MG Vander Heiden","year":"2009","unstructured":"Vander Heiden, M. G., Cantley, L. C. & Thompson, C. B. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 324, 1029\u20131033 (2009).","journal-title":"Science."},{"key":"66607_CR32","doi-asserted-by":"publisher","first-page":"E293","DOI":"10.3390\/cells8040293","volume":"8","author":"E Mik\u00f3","year":"2019","unstructured":"Mik\u00f3, E. et al. Microbiome-Microbial Metabolome-Cancer Cell Interactions in Breast Cancer-Familiar, but Unexplored. Cells. 8, E293, https:\/\/doi.org\/10.3390\/cells8040293 (2019).","journal-title":"Cells."},{"key":"66607_CR33","doi-asserted-by":"publisher","first-page":"992","DOI":"10.1038\/ncb3039","volume":"16","author":"VS LeBleu","year":"2014","unstructured":"LeBleu, V. S. et al. PGC-1\u03b1 mediates mitochondrial biogenesis and oxidative phosphorylation in cancer cells to promote metastasis. Nat. Cell Biol. 16, 992\u20131003 (2014).","journal-title":"Nat. Cell Biol."},{"key":"66607_CR34","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.3892\/ol.2012.928","volume":"4","author":"J Zheng","year":"2012","unstructured":"Zheng, J. Energy metabolism of cancer: Glycolysis versus oxidative phosphorylation. Oncol. Lett 4, 1151\u20131157 (2012).","journal-title":"Oncol. Lett"},{"key":"66607_CR35","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1007\/s11306-019-1555-9","volume":"15","author":"VL Kouznetsova","year":"2019","unstructured":"Kouznetsova, V. L. et al. Recognition of early and late stages of bladder cancer using metabolites and machine learning. Metabolomics. 15, 94\u2013108 (2019).","journal-title":"Metabolomics."},{"key":"66607_CR36","doi-asserted-by":"publisher","first-page":"5597","DOI":"10.1158\/0008-5472.CAN-04-0603","volume":"64","author":"J Yang","year":"2004","unstructured":"Yang, J., Chen, Z., Liu, Y., Hickey, R. J. & Malkas, L. H. Altered DNA polymerase \u03b9 expression in breast cancer cells leads to a reduction in DNA replication fidelity and a higher rate of mutagenesis. Cancer Res. 64, 5597\u20135607 (2004).","journal-title":"Cancer Res."},{"key":"66607_CR37","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/j.chom.2012.02.006","volume":"11","author":"MA von Schillde","year":"2012","unstructured":"von Schillde, M. A. et al. Lactocepin secreted by Lactobacillus exerts anti-inflammatory effects by selectively degrading proinflammatory chemokines. Cell host microbe 11, 387\u2013396 (2012).","journal-title":"Cell host microbe"},{"key":"66607_CR38","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1111\/cas.13497","volume":"109","author":"H Song","year":"2018","unstructured":"Song, H. et al. Pretreatment with probiotic Bifico ameliorates colitis\u2010associated cancer in mice: Transcriptome and gut flora profiling. Cancer Sci. 109, 666\u2013677 (2018).","journal-title":"Cancer Sci."},{"key":"66607_CR39","doi-asserted-by":"publisher","first-page":"11855","DOI":"10.1073\/pnas.90.24.11855","volume":"90","author":"Z Matijasevic","year":"1993","unstructured":"Matijasevic, Z., Boosalis, M., Mackay, W., Samson, L. & Ludlum, D. B. Protection against chloroethylnitrosourea cytotoxicity by eukaryotic 3-methyladenine DNA glycosylase. Proc. Nati. Acad. Sci. U S A. 90, 11855\u201311859 (1993).","journal-title":"Proc. Nati. Acad. Sci. U S A."},{"key":"66607_CR40","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1002\/j.1460-2075.1996.tb00429.x","volume":"15","author":"BP Engelward","year":"1996","unstructured":"Engelward, B. P. et al. Repair\u2010deficient 3\u2010methyladenine DNA glycosylase homozygous mutant mouse cells have increased sensitivity to alkylation\u2010induced chromosome damage and cell killing. EMBO J. 15, 945\u2013952 (1996).","journal-title":"EMBO J."},{"key":"66607_CR41","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1016\/S1568-7864(03)00087-9","volume":"2","author":"S Adimoolam","year":"2003","unstructured":"Adimoolam, S. & Ford, J. M. p53 and regulation of DNA damage recognition during nucleotide excision repair. DNA repair (Amst) 2, 947\u2013954 (2003).","journal-title":"DNA repair (Amst)"},{"key":"66607_CR42","doi-asserted-by":"publisher","first-page":"4598","DOI":"10.1093\/nar\/29.22.4598","volume":"29","author":"CT Nguyen","year":"2001","unstructured":"Nguyen, C. T., Gonzales, F. A. & Jones, P. A. Altered chromatin structure associated with methylation-induced gene silencing in cancer cells: correlation of accessibility, methylation, MeCP2 binding and acetylation. Nucleic Acids Res 29, 4598\u20134606 (2001).","journal-title":"Nucleic Acids Res"},{"key":"66607_CR43","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1038\/ng1068","volume":"33","author":"MF Robert","year":"2002","unstructured":"Robert, M. F. et al. DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells. Nat. Genet. 33, 61\u201365 (2002).","journal-title":"Nat. Genet."},{"key":"66607_CR44","doi-asserted-by":"crossref","unstructured":"Oh, H. Y. et al. The association of uterine cervical microbiota with an increased risk for cervical intraepithelial neoplasia in Korea. Clin. Microbiol. Infect. 21,\u00a0674.e1\u2013674.e9 (2015).","DOI":"10.1016\/j.cmi.2015.02.026"},{"key":"66607_CR45","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1038\/nrmicro3330","volume":"12","author":"P Yarza","year":"2014","unstructured":"Yarza, P. et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat. Rev. Microbiol. 12, 635\u2013645 (2014).","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-66607-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-66607-4","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-66607-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T07:18:21Z","timestamp":1670311101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-66607-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,16]]},"references-count":45,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["66607"],"URL":"https:\/\/doi.org\/10.1038\/s41598-020-66607-4","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,16]]},"assertion":[{"value":"3 December 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 May 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 June 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"9720"}}