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This study established an EB mouse model with all the above features. First, 42 mice were divided into 7 groups to investigate the optimal time interval between cough and AHR detections. Afterward, 28 mice were divided into the asthma, EB, normal saline (NS), and dexamethasone (DXM) groups. Mice were challenged using nasal drops of 200\u00a0\u00b5g ovalbumin (OVA), 10\u00a0\u00b5g OVA, NS, or intraperitoneal injections of 5\u00a0mg\/kg of DXM one hour prior to 10\u00a0\u00b5g OVA challenge. Airway reactivity was measured 6\u00a0h after cough was observed. The frequency of coughs in the asthma and EB groups increased significantly compared to mice in the NS group. After DXM administration, frequency of coughs was significantly decreased compared to mice in the asthma and EB groups. Lung resistance in the asthma group was significantly higher compared to mice in the NS, EB, and DXM groups. Obvious airway eosinophilic inflammation in BALF and lung tissues were observed in the asthma and EB groups, while DXM administration could attenuate airway inflammatory infiltration. In summary, we developed a mouse EB model with all four clinical features of EB by the administration of 10\u00a0\u00b5g OVA nasal drops.<\/jats:p>","DOI":"10.1038\/s41598-020-67475-8","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T10:03:01Z","timestamp":1593424981000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Establishment of a mouse model with all four clinical features of eosinophilic bronchitis"],"prefix":"10.1038","volume":"10","author":[{"given":"Liyan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenhui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxing","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kefang","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanshan","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"issue":"8651","key":"67475_CR1","doi-asserted-by":"publisher","first-page":"1346","DOI":"10.1016\/S0140-6736(89)92801-8","volume":"333","author":"PG Gibson","year":"1989","unstructured":"Gibson, P. 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