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Various doubts have already appeared in preliminary stages of microbiological diagnostics of <jats:italic>Haemophilus<\/jats:italic> spp. A total of 87 <jats:italic>H. parainfluenzae<\/jats:italic> isolates were obtained from throat or nasopharyngeal swabs from adults 18 to 70\u00a0years old, both healthy volunteers and patients with chronic diseases between 2013 to 2015 in eastern Poland. <jats:italic>Haemophilus<\/jats:italic> spp. were identified by colony morphology, Gram-staining, API NH and MALDI-TOF MS technique. Both susceptibility to various antimicrobials and phenotypes of <jats:italic>Haemophilus<\/jats:italic> spp. resistance to beta-lactams were determined. Statistically significant association between applied guidelines and drug resistance patterns were observed to as follows: ampicillin, cefuroxime, cefotaxime, amoxicillin-clavulanate, azithromycin, tetracycline and trimethoprim-sulfamethoxazole. Resistance phenotypes according to CLSI vs. EUCAST were as follows: 3.4% vs. 8.0% for BLNAR and 6.9% vs. 19.5% for BLPACR isolates. In conclusion, this is the first study that reports comparative analysis of drug susceptibility interpretation using CLSI and EUCAST of haemophili rods from human respiratory microbiota in Poland. In case of susceptible, increased exposure (formerly intermediate) category of susceptibility within <jats:italic>H. parainfluenzae<\/jats:italic> isolates we have observed EUCAST as more restrictive than CLSI. Moreover, BLNAI and BLPAI phenotype isolates have been observed, as well as BLPBR using only CLSI or EUCAST guidelines, respectively.<\/jats:p>","DOI":"10.1038\/s41598-020-68161-5","type":"journal-article","created":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T10:04:30Z","timestamp":1594634670000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence of susceptibility patterns of opportunistic bacteria in line with CLSI or EUCAST among Haemophilus parainfluenzae isolated from respiratory microbiota"],"prefix":"10.1038","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4536-1750","authenticated-orcid":false,"given":"Urszula","family":"Kosikowska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6301-6059","authenticated-orcid":false,"given":"Sylwia","family":"Andrzejczuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0451-4741","authenticated-orcid":false,"given":"Ewelina","family":"Grywalska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edyta","family":"Chwiejczak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8468-2154","authenticated-orcid":false,"given":"Stanis\u0142aw","family":"Winiarczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Pietras-O\u017cga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5238-1007","authenticated-orcid":false,"given":"Dagmara","family":"St\u0119pie\u0144-Py\u015bniak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"68161_CR1","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1164\/rccm.201407-1240OC","volume":"190","author":"G Biesbroek","year":"2014","unstructured":"Biesbroek, G. et al. 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