{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T11:27:53Z","timestamp":1793014073989,"version":"4.5.0"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Public-Private Innovation Program of MEXT"},{"name":"International Joint Research Promotion program of Osaka University"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of blood during ophthalmic surgery is problematic, as it can obstruct a surgeon\u2019s view of the operative field. This is particularly true when performing trabeculectomy surgery to enhance ocular fluid outflow and reduce intraocular pressure as a treatment for glaucoma, one of the most common vision loss conditions worldwide. In this study, we investigated the performance of a transparent, self-assembling peptide gel (SPG-178) and its ability to maintain visibility during trabeculectomy surgery. Unlike the hyaluronic acid gel commonly used in ophthalmic surgery, SPG-178 did not permit the ingress of blood into the gel itself. Rather, it forced blood to flow peripherally to the gel. Moreover, if bleeding occurred under the SPG-178 gel, perfusion with saline was able to effectively flush the blood away along the interface between the SPG-178 and the ocular tissue (in this case scleral) to clear the surgical field of view. In experimental trabeculectomy surgeries with mitomycin C used as an adjuvant, there were no differences in the postoperative recovery of intraocular\u00a0pressure or bleb morphology with or without the use of SPG-178. SPG-178, therefore, when used in a gel formulation, represents a new material for use in intraocular surgery to ensure a clear operative field with likely beneficial treatment outcomes.<\/jats:p>","DOI":"10.1038\/s41598-020-68171-3","type":"journal-article","created":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T10:06:38Z","timestamp":1594289198000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of a self-assembling peptide gel, SPG-178, in providing a clear operative field for trabeculectomy surgery for glaucoma in an animal model"],"prefix":"10.1038","volume":"10","author":[{"given":"Kenji","family":"Matsushita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumi","family":"Kawashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Uesugi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruka","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Sakaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Quantock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohji","family":"Nishida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,9]]},"reference":[{"key":"68171_CR1","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1007\/s003810050225","volume":"14","author":"KC Wang","year":"1998","unstructured":"Wang, K. C., Cho, B. K., Kim, C. S. & Kim, S. D. Control of intraoperative bleeding with hydrostatic pressure during endoscopic. Childs. Nerv. Syst. 14, 280\u2013284 (1998).","journal-title":"Childs. Nerv. Syst."},{"key":"68171_CR2","doi-asserted-by":"publisher","first-page":"e104477","DOI":"10.1371\/journal.pone.0104477","volume":"9","author":"J Jahanshahi","year":"2014","unstructured":"Jahanshahi, J. et al. Effect of topical tranexamic acid on bleeding and quality of surgical field during functional endoscopic sinus surgery in patients with chronic rhinosinusitis: a triple blind randomized clinical trial. PLoS ONE 9, e104477 (2014).","journal-title":"PLoS ONE"},{"key":"68171_CR3","first-page":"618","volume":"12","author":"M Ochiai","year":"2000","unstructured":"Ochiai, M. et al. Efficacy of a new hemostatic device, adapty, after transradial coronary angiofraphy and intervenetion. J. Invative Cardiol. 12, 618\u2013622 (2000).","journal-title":"J. Invative Cardiol."},{"key":"68171_CR4","doi-asserted-by":"publisher","first-page":"e102778","DOI":"10.1371\/journal.pone.0102778","volume":"9","author":"S Komatsu","year":"2014","unstructured":"Komatsu, S. et al. The neutral self-assembling peptide hydrogel SPG-178 as a topical hemostatic agent. PLoS ONE 9, e102778 (2014).","journal-title":"PLoS ONE"},{"key":"68171_CR5","doi-asserted-by":"publisher","first-page":"2357","DOI":"10.1039\/C7BM00554G","volume":"5","author":"X Yang","year":"2017","unstructured":"Yang, X. et al. Design and development of polysaccharide hemostatic materials and their hemostatic mechanism. Biomater. Sci. 5, 2357\u20132368 (2017).","journal-title":"Biomater. Sci."},{"key":"68171_CR6","doi-asserted-by":"publisher","first-page":"445","DOI":"10.5761\/atcs.oa.12.01977","volume":"18","author":"H Masuhara","year":"2012","unstructured":"Masuhara, H., Fujii, T., Watanabe, Y., Koyama, N. & Tokuhiro, K. Novel infections agent-free hemostatic material (TDM-621) in cardiovascular surgery. Ann. Thorac. Cardiovasc. Surg. 18, 445\u2013451 (2012).","journal-title":"Ann. Thorac. Cardiovasc. Surg."},{"key":"68171_CR7","first-page":"1","volume-title":"Eye Surgery: An Introduction to Operative Technique","author":"G Eisner","year":"2012","unstructured":"Eisner, G. Eye Surgery: An Introduction to Operative Technique 1\u201356 (Springer, New York, 2012)."},{"key":"68171_CR8","doi-asserted-by":"publisher","first-page":"131","DOI":"10.4103\/0301-4738.73689","volume":"59","author":"L Vijiaya","year":"2011","unstructured":"Vijiaya, L., Manis, P., Ronnie, G. & Shantha, S. Management of complications in glaucoma surgery. Indian J. Ophthalmol. 59, 131\u2013140 (2011).","journal-title":"Indian J. Ophthalmol."},{"key":"68171_CR9","first-page":"844","volume":"82","author":"S Resniloff","year":"2004","unstructured":"Resniloff, S. et al. Global date on visual impairment in the year 2002. Bull. World Health Organ. 82, 844\u2013851 (2004).","journal-title":"Bull. World Health Organ."},{"key":"68171_CR10","first-page":"887","volume":"82","author":"S Kingman","year":"2004","unstructured":"Kingman, S. Glaucoma is second leading cause of blindness globally. Bull. World Health Organ. 82, 887\u2013888 (2004).","journal-title":"Bull. World Health Organ."},{"key":"68171_CR11","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1016\/j.biomaterials.2011.10.049","volume":"33","author":"Y Nagai","year":"2012","unstructured":"Nagai, Y., Yokoi, H., Kaihara, K. & Naruse, K. The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel. Biomaterials 33, 1044\u20131051 (2012).","journal-title":"Biomaterials"},{"key":"68171_CR12","doi-asserted-by":"publisher","first-page":"1201","DOI":"10.1097\/BRS.0000000000001611","volume":"41","author":"K Ando","year":"2016","unstructured":"Ando, K. et al. Self-assembling peptide reduces glial scarring, attenuates posttraumatic inflammation, and promotes neurite outgrowth of spinal motor neurons. Spine 41, 1201\u20131207 (2016).","journal-title":"Spine"},{"key":"68171_CR13","doi-asserted-by":"publisher","first-page":"1394","DOI":"10.1089\/ten.tea.2017.0025","volume":"23","author":"J Tsukamoto","year":"2017","unstructured":"Tsukamoto, J. et al. Efficacy of a self-assembling peptide hydrogel, SPG-178-gel, for bone regeneration and three-dimensional osteogenic induction of dental pulp stem cells. Tissue Eng. A 23, 1394\u20131402 (2017).","journal-title":"Tissue Eng. A"},{"key":"68171_CR14","doi-asserted-by":"publisher","first-page":"4068","DOI":"10.1167\/iovs.17-21536","volume":"58","author":"K Uesugi","year":"2017","unstructured":"Uesugi, K. et al. A self-assembling peptide gel as a vitreous substitute: a rabbit study. Invest. Ophthalmol. Vis. Sci. 58, 4068\u20134075 (2017).","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"68171_CR15","doi-asserted-by":"publisher","first-page":"e0190833","DOI":"10.1371\/journal.pone.0190833","volume":"13","author":"K Ando","year":"2018","unstructured":"Ando, K. et al. Effect of self-assembling peptide as a scaffold on bone formation in a defect. PLoS ONE 13, e0190833 (2018).","journal-title":"PLoS ONE"},{"key":"68171_CR16","first-page":"172","volume":"9","author":"CW Chen","year":"1983","unstructured":"Chen, C. W., Huang, H. T., Bair, J. S. & Lee, C. C. Enhanced intraocular pressure controlling effectiveness of trabeculectomy by local application of mitomycin C. Trans. Asia Pacific Acad. Ophthalmol. 9, 172\u2013177 (1983).","journal-title":"Trans. Asia Pacific Acad. Ophthalmol."},{"key":"68171_CR17","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1089\/jop.1990.6.175","volume":"6","author":"CW Chen","year":"1990","unstructured":"Chen, C. W., Huang, H. T., Bair, J. S. & Lee, C. C. Trabeculectomy with simultaneous topical application of mitomycin C in refractory glaucoma. J. Ocul. Pharmacol. 6, 175\u2013182 (1990).","journal-title":"J. Ocul. Pharmacol."},{"key":"68171_CR18","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/S0161-6420(91)32293-0","volume":"99","author":"SS Palmer","year":"1991","unstructured":"Palmer, S. S. Mitomycin as adjuuctive chemotherapy with trabeculectomy. Ophthalmology 99, 317\u2013321 (1991).","journal-title":"Ophthalmology"},{"key":"68171_CR19","doi-asserted-by":"publisher","first-page":"1693","DOI":"10.1001\/archopht.1991.01080120077030","volume":"109","author":"Y Kitazawa","year":"1991","unstructured":"Kitazawa, Y., Kawase, K., Matsushita, H. & Minobe, M. Trabeculectomy with mitomycin: comparative study with fluorouracil. Arch. Ophthalmol. 109, 1693\u20131698 (1991).","journal-title":"Arch. Ophthalmol."},{"key":"68171_CR20","unstructured":"Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https:\/\/imagej.nih.gov\/ij\/, 1997\u20132016."},{"key":"68171_CR21","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1136\/bjo.35.4.232","volume":"35","author":"DP Greaves","year":"1951","unstructured":"Greaves, D. P. & Perkins, E. S. Buphthalmos in the rabbit. Br. J. Ophthalmol. 35, 232\u2013233 (1951).","journal-title":"Br. J. Ophthalmol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-68171-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-68171-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-68171-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T13:49:13Z","timestamp":1670334553000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-020-68171-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,9]]},"references-count":21,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["68171"],"URL":"https:\/\/doi.org\/10.1038\/s41598-020-68171-3","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,9]]},"assertion":[{"value":"11 February 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 June 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 July 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Financial Support: Menicon (K.N., H.S.), Employment: Menicon (K.U., H.O.), and Patent: Menicon (K.N., K.M., H.S., K.U.). The other authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"11326"}}