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Here, we investigated genome-wide DNA methylation profiles specifically associated with DCI, which is a major contributor to poor clinical outcomes. An epigenome-wide association study (EWAS) and quantitative real-time PCR (qRT-PCR) were conducted in 40 SAH patients (DCI, n\u2009=\u200913; non-DCI, n\u2009=\u200927). A replication study using bisulfite modification and methylation-specific PCR was further performed in 36 patients (DCI, n\u2009=\u200912; non-DCI, n\u2009=\u200924). The relative degree of methylation was described as the median and 25th\u201375th percentile. No significant differences in clinical characteristics between DCI and non-DCI groups were observed. Among the top 10 differentially methylated genes analyzed via EWAS, two aberrantly methylated CpG sites of cg00441765 (<jats:italic>INSR<\/jats:italic> gene) and cg11464053 (<jats:italic>CDHR5<\/jats:italic> gene) were associated with decreased mRNA expression (2<jats:sup>\u2212\u0394Ct<\/jats:sup>). They include <jats:italic>INSR<\/jats:italic> [0.00020 (0.00012\u20130.00030) in DCI vs. 0.00050 (0.00030\u20130.00068) in non-DCI] and <jats:italic>CDHR5<\/jats:italic> [0.114 (0.053\u20130.143) in DCI vs. 0.170 (0.110\u20130.212) in non-DCI]. Compared with non-DCI cases, patients with DCI exhibited an increased degree of methylation in the replication study: <jats:italic>INSR<\/jats:italic>, 0.855 (0.779\u20130.913) in DCI vs. 0.582 (0.565\u20130.689) in non-DCI; <jats:italic>CDHR5<\/jats:italic>, 0.786 (0.708\u20130.904) in DCI vs. 0.632 (0.610\u20130.679) in non-DCI. Hypermethylation of two novel genes, <jats:italic>INSR<\/jats:italic> and <jats:italic>CDHR5<\/jats:italic> may serve as a biomarker for early detection of DCI following SAH.<\/jats:p>","DOI":"10.1038\/s41598-020-68325-3","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T10:04:06Z","timestamp":1594375446000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Genome-wide blood DNA methylation analysis in patients with delayed cerebral ischemia after subarachnoid hemorrhage"],"prefix":"10.1038","volume":"10","author":[{"given":"Bong Jun","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngmi","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Hyuk","family":"Youn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Jin","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Kook","family":"Rhim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heung Cheol","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keunsoo","family":"Kang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Pyeong","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,10]]},"reference":[{"key":"68325_CR1","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1159\/000487325","volume":"50","author":"W Duan","year":"2018","unstructured":"Duan, W. et al. 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