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Hauck Associate Director endowment for the Center for Individualized Medicine, Mayo Clinic"},{"name":"William O. Lund, Jr. and Natalie C. Lund Charitable Foundation endowment for the Center for Individualized Medicine, Mayo Clinic"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The relationship between primary biliary cholangitis (PBC), a chronic cholestatic autoimmune liver disease, and the peripheral immune system remains to be fully understood. Herein, we performed the first mass cytometry (CyTOF)-based, immunophenotyping analysis of the peripheral immune system in PBC at single-cell resolution. CyTOF was performed on peripheral blood mononuclear cells (PBMCs) from PBC patients (n\u2009=\u200933) and age-\/sex-matched healthy controls (n\u2009=\u200933) to obtain immune cell abundance and marker expression profiles. Hierarchical clustering methods were applied to identify immune cell types and subsets significantly associated with PBC. Subsets of gamma-delta T cells (CD3\n                    <jats:sup>+<\/jats:sup>\n                    TCRgd\n                    <jats:sup>+<\/jats:sup>\n                    ), CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells (CD3\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    CD161\n                    <jats:sup>+<\/jats:sup>\n                    PD1\n                    <jats:sup>+<\/jats:sup>\n                    ), and memory B cells (CD3\n                    <jats:sup>\u2212<\/jats:sup>\n                    CD19\n                    <jats:sup>+<\/jats:sup>\n                    CD20\n                    <jats:sup>+<\/jats:sup>\n                    CD24\n                    <jats:sup>+<\/jats:sup>\n                    CD27\n                    <jats:sup>+<\/jats:sup>\n                    ) were found to have lower abundance in PBC than in control. In contrast, higher abundance of subsets of monocytes and na\u00efve B cells were observed in PBC compared to control. Furthermore, several na\u00efve B cell (CD3\n                    <jats:sup>\u2212<\/jats:sup>\n                    CD19\n                    <jats:sup>+<\/jats:sup>\n                    CD20\n                    <jats:sup>+<\/jats:sup>\n                    CD24\n                    <jats:sup>\u2212<\/jats:sup>\n                    CD27\n                    <jats:sup>\u2212<\/jats:sup>\n                    ) subsets were significantly higher in PBC patients with cirrhosis (indicative of late-stage disease) than in those without cirrhosis. Alternatively, subsets of memory B cells were lower in abundance in cirrhotic relative to non-cirrhotic PBC patients. Future immunophenotyping investigations could lead to better understanding of PBC pathogenesis and progression, and also to the discovery of novel biomarkers and treatment strategies.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-69358-4","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T06:04:50Z","timestamp":1595916290000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Single-cell mass cytometry on peripheral blood identifies immune cell subsets associated with primary biliary cholangitis"],"prefix":"10.1038","volume":"10","author":[{"given":"Jin Sung","family":"Jang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian D.","family":"Juran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin Y.","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinod K.","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Min","family":"Son","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju Dong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad H.","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Ann L.","family":"Enninga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaeyun","family":"Sung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos N.","family":"Lazaridis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,28]]},"reference":[{"key":"69358_CR1","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1038\/nrgastro.2016.201","volume":"14","author":"GJ Webb","year":"2017","unstructured":"Webb, G. 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