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P4-ATPases (flippases) are involved in the generation of such asymmetry. In <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, the protein kinases Fpk1p\/Fpk2p activate the P4-ATPases Dnf1p\/Dnf2p by phosphorylation. Previously, we have shown that a blue-light-dependent protein kinase, phototropin from <jats:italic>Chlamydomonas reinhardtii<\/jats:italic> (<jats:italic>Cr<\/jats:italic>PHOT), complements defects in an <jats:italic>fpk1<\/jats:italic>\u0394 <jats:italic>fpk2<\/jats:italic>\u0394 mutant. Herein, we investigated whether <jats:italic>Cr<\/jats:italic>PHOT optically regulates P4-ATPase activity. First, we demonstrated that the translocation of NBD-labelled phospholipids to the cytoplasmic leaflet via P4-ATPases was promoted by blue-light irradiation in <jats:italic>fpk1<\/jats:italic>\u0394 <jats:italic>fpk2<\/jats:italic>\u0394 cells with <jats:italic>Cr<\/jats:italic>PHOT. In addition, blue light completely suppressed the defects in membrane functions (such as endocytic recycling, actin depolarization, and apical-isotropic growth switching) caused by <jats:italic>fpk1<\/jats:italic>\u0394 <jats:italic>fpk2<\/jats:italic>\u0394 mutations. All responses required the kinase activity of <jats:italic>Cr<\/jats:italic>PHOT. Hence, these results indicate the utility of <jats:italic>Cr<\/jats:italic>PHOT as a powerful and first tool for optogenetic manipulation of P4-ATPase activity.<\/jats:p>","DOI":"10.1038\/s41598-020-69459-0","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T06:03:22Z","timestamp":1595829802000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast"],"prefix":"10.1038","volume":"10","author":[{"given":"Tomomi","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Mioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuma","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Nagatani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,7,27]]},"reference":[{"key":"69459_CR1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj2940001","volume":"294","author":"A Zachowski","year":"1993","unstructured":"Zachowski, A. 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