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Maintenance of complex barrier functions is achieved through anatomical structures in the skin, the stratified squamous epithelium specialized junctional organelles, called tight junctions (TJs). Several members of our microbial communities are known to affect the differentiation state and function of the colonized organ. Whether and how interactions between skin cells and cutaneous microbes, including <jats:italic>Cutibacterium acnes<\/jats:italic> (<jats:italic>C. acnes<\/jats:italic>), modify the structure and\/or function of our skin is currently only partly understood. Thus, in our studies, we investigated whether <jats:italic>C. acnes<\/jats:italic> may affect the epidermal barrier using in vitro model systems. Real-time cellular analysis showed that depending on the keratinocyte differentiation state, the applied <jats:italic>C. acnes<\/jats:italic> strains and their dose, the measured impedance values change, together with the expression of selected TJ proteins. These may reflect barrier alterations, which can be partially restored upon antibiotic\u2013antimycotic treatment. Our findings suggest that <jats:italic>C. acnes<\/jats:italic> can actively modify the barrier properties of cultured keratinocytes, possibly through alteration of tight cell-to-cell contacts. Similar events may play important roles in our skin, in the maintenance of cutaneous homeostasis.<\/jats:p>","DOI":"10.1038\/s41598-020-69677-6","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T10:24:33Z","timestamp":1596104673000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Cutibacterium acnes regulates the epidermal barrier properties of HPV-KER human immortalized keratinocyte cultures"],"prefix":"10.1038","volume":"10","author":[{"given":"Be\u00e1ta Szilvia","family":"Bolla","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilla","family":"Erdei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edit","family":"Urb\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katalin","family":"Buri\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lajos","family":"Kem\u00e9ny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korn\u00e9lia","family":"Szab\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"69677_CR1","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1111\/j.1600-0625.2008.00786.x","volume":"17","author":"E Proksch","year":"2008","unstructured":"Proksch, E., Brandner, J. 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