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This study was designed to determine the frequency of <jats:italic>Plasmodium falciparum<\/jats:italic> isolates with histidine-rich protein 2 (<jats:italic>pfhrp2<\/jats:italic>) gene deletion as one of the possible factors contributing to the failure of PfHRP2-based RDTs in detecting malaria. A total of 300 blood samples were collected from several health centres in Nyala City, Western Sudan. The performance of PfHRP2-based RDTs in relation to microscopy was examined and the PCR-confirmed samples were investigated for the presence of <jats:italic>pfhrp2<\/jats:italic> gene. A total of 113 out of 300 patients were <jats:italic>P. falciparum<\/jats:italic> positive by microscopy. Among them, 93.81% (106 out of 113) were positives by the PfHRP2 RDTs. Seven isolates were identified as false negative on the basis of the RDTs results. Only one isolate (0.9%; 1\/113) potentially has <jats:italic>pfhrp2<\/jats:italic> gene deletion. The sensitivity and specificity of PfHRP2-based RDTs were 93.81% and 100%, respectively. The results provide insights into the <jats:italic>pfhrp2<\/jats:italic> gene deletion amongst <jats:italic>P. falciparum<\/jats:italic> population from Sudan. However, further studies with a large and systematic collection from different geographical settings across the country are needed.<\/jats:p>","DOI":"10.1038\/s41598-020-69756-8","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T10:24:33Z","timestamp":1596104673000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Plasmodium falciparum isolate with histidine-rich protein 2 gene deletion from Nyala City, Western Sudan"],"prefix":"10.1038","volume":"10","author":[{"given":"Mohammed A.","family":"Boush","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moussa A.","family":"Djibrine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Mussa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Talib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Maki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrahman","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid B.","family":"Beshir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeehaida","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid","family":"Hajissa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"69756_CR1","doi-asserted-by":"publisher","first-page":"e0206303","DOI":"10.1371\/journal.pone.0206303","volume":"13","author":"KA Adusei","year":"2018","unstructured":"Adusei, K. 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