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They constitute a large protein family of receptors with almost 300 members detected in human pancreatic islet preparations. However, the functional role of these receptors in pancreatic islets is unknown in most cases. We generated a new stable human beta cell line from neonatal pancreas. This cell line, named ECN90 expresses both subunits (<jats:italic>GABBR1<\/jats:italic> and <jats:italic>GABBR2<\/jats:italic>) of the metabotropic GABA<jats:sub>B<\/jats:sub> receptor compared to human islet. In ECN90 cells, baclofen, a specific GABA<jats:sub>B<\/jats:sub> receptor agonist, inhibits cAMP signaling causing decreased expression of beta cell-specific genes such as <jats:italic>MAFA<\/jats:italic> and <jats:italic>PCSK1,<\/jats:italic> and reduced insulin secretion. We next demonstrated that in primary human islets, <jats:italic>GABBR2<\/jats:italic> mRNA expression is strongly induced under cAMP signaling, while <jats:italic>GABBR1<\/jats:italic> mRNA is constitutively expressed. We also found that induction and activation of the GABA<jats:sub>B<\/jats:sub> receptor in human islets modulates insulin secretion.<\/jats:p>","DOI":"10.1038\/s41598-020-69758-6","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T10:03:08Z","timestamp":1597053788000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Regulated expression and function of the GABAB receptor in human pancreatic beta cell line and islets"],"prefix":"10.1038","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1089-2837","authenticated-orcid":false,"given":"Latif","family":"Rachdi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Maugein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Severine","family":"Pechberty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Armanet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette","family":"Hamroune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Ravassard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Marullo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Albagli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Scharfmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"69758_CR1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.arcmed.2005.01.003","volume":"36","author":"JL Leahy","year":"2005","unstructured":"Leahy, J. 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