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In the absence of vaccines, control of fascioliasis is primarily via anthelminthic treatment with triclabendazole (TCBZ). Parasitic flatworms, including <jats:italic>Fasciola hepatica,<\/jats:italic> are active secretors of extracellular vesicles (EVs), but research has not been undertaken investigating EV anthelmintic sequestration. Adult <jats:italic>F. hepatica<\/jats:italic> were cultured in lethal and sub-lethal doses of TCBZ and its active metabolites, in order to collect EVs and evaluate their morphological characteristics, production and anthelmintic metabolite content. Transmission electron microscopy demonstrated that <jats:italic>F. hepatica<\/jats:italic> exposed to TCBZ and its metabolites produced EVs of similar morphology, compared to non-TCBZ exposed controls, even though TCBZ dose and\/or TCBZ metabolite led to measurable structural changes in the treated <jats:italic>F. hepatica<\/jats:italic> tegument. qNano particle analysis revealed that <jats:italic>F. hepatica<\/jats:italic> exposed to TCBZ and its metabolites produced at least five times greater EV concentrations than non-TCBZ controls. A combined mass spectrometry and qNano particle analysis confirmed the presence of TCBZ and the TCBZ\u2013sulphoxide metabolite in anthelmintic exposed EVs, but limited TCBZ sulphone was detectable. This data suggests that EVs released from adult <jats:italic>F. hepatica<\/jats:italic> have a biological role in the sequestration of TCBZ and additional toxic xenobiotic metabolites.<\/jats:p>","DOI":"10.1038\/s41598-020-69970-4","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T10:03:08Z","timestamp":1597053788000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Evidence of sequestration of triclabendazole and associated metabolites by extracellular vesicles of Fasciola hepatica"],"prefix":"10.1038","volume":"10","author":[{"given":"Chelsea N.","family":"Davis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Winters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Milic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Devitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Cookson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Brophy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell M.","family":"Morphew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"69970_CR1","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1017\/S0031182013001662","volume":"141","author":"J Charlier","year":"2014","unstructured":"Charlier, J., Vercruysse, J., Morgan, E., Van Dijk, J. & Williams, D. 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