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Therefore, it is highly necessary to advance the development of novel antimicrobial compounds and semen preservation strategies. The aim of this study was to evaluate a low temperature, antibiotic-free preservation procedure using Androstar Premium (ASP) extender (Minit\u00fcb) with antimicrobial lipopeptides. Firstly, seven lipopeptides in two concentrations (1\u2009\u00d7\u2009minimum inhibitory concentration (MIC)\/2\u2009\u00d7\u2009MIC) were tested on their sperm-compatibility at 17\u00a0\u00b0C. Two lipopeptides, C16-KKK-NH<jats:sub>2<\/jats:sub> and C16-KKKK-NH<jats:sub>2<\/jats:sub>, did not negatively affect sperm quality and were further evaluated for their efficiency of bacterial growth inhibition at 5\u00a0\u00b0C. Besides an overall diminution of colony forming units, both peptides showed a reduction of bacterial subcultures (n\u2009=\u2009103) with a decrement in Gram-positive rods from 65 (ASP w\/o supplements) to 39\/52 (ASP w\/ C16-KKK-NH<jats:sub>2<\/jats:sub>\/C16-KKKK-NH<jats:sub>2<\/jats:sub>), in Gram-positive cocci from 21 to 9\/10 and in Gram-negative species from 17 to 8\/5 total subcultures. Furthermore, lipopeptides revealed activity towards selected bacteria of potential concern in artificial insemination like <jats:italic>Trueperella pyogenes<\/jats:italic>, <jats:italic>Alcaligenes faecalis<\/jats:italic>, <jats:italic>Pseudomonas aeruginosa<\/jats:italic> (not C16-KKK-NH<jats:sub>2<\/jats:sub>), <jats:italic>Pasteurella<\/jats:italic> sp., <jats:italic>Providencia stuartii<\/jats:italic>, <jats:italic>Escherichia coli<\/jats:italic> (not C16-KKKK-NH<jats:sub>2<\/jats:sub>) and <jats:italic>Streptococcus porcinus<\/jats:italic> (not C16-KKKK-NH<jats:sub>2<\/jats:sub>). Consequently, both tested lipopeptides are promising candidates for alternative antibiotic-free preservation techniques of boar semen.<\/jats:p>","DOI":"10.1038\/s41598-020-70180-1","type":"journal-article","created":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T10:04:51Z","timestamp":1596708291000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load"],"prefix":"10.1038","volume":"10","author":[{"given":"B.","family":"Hensel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Jakop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Scheinpflug","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"M\u00fchldorfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Schr\u00f6ter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Sch\u00e4fer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Greber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Schulze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,6]]},"reference":[{"key":"70180_CR1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1179\/2047773215Y.0000000030","volume":"109","author":"F Prestinaci","year":"2015","unstructured":"Prestinaci, F., Pezzotti, P. & Pantosti, A. 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