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Prolargin\u2014an extracellular matrix (ECM) protein present in cartilage and tendon\u2014has been previously shown elevated in serum of patients with psoriasis. ECM protein fragments can reflect tissue turnover and pathological changes; thus, this study aimed to develop, validate and characterize a novel biomarker PROM targeting a matrix metalloproteinase (MMP)-cleaved prolargin neo-epitope, and to evaluate it as a biomarker for PsA. A competitive ELISA was developed with a monoclonal mouse antibody; dilution- and spiking-recovery, inter- and intra-variation, and accuracy were evaluated. Serum levels were evaluated in 55 healthy individuals and 111 patients diagnosed with PsA by the CASPAR criteria. Results indicated that the PROM assay was specific for the neo-epitope. Inter- and intra- assay variations were 11% and 4%, respectively. PROM was elevated (p\u2009=\u20090.0003) in patients with PsA (median: 0.24, IQR: 0.19\u20130.31) compared to healthy controls (0.18; 0.14\u20130.23) at baseline. AUROC for separation of healthy controls from PsA patients was 0.674 (95% CI 0.597\u20130.744, P\u2009&lt;\u20090.001). In conclusion, MMP-cleaved prolargin can be quantified in serum by the PROM assay and has the potential to separate patients with PsA from healthy controls.<\/jats:p>","DOI":"10.1038\/s41598-020-70327-0","type":"journal-article","created":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T06:10:29Z","timestamp":1597126229000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A novel biomarker of MMP-cleaved prolargin is elevated in patients with psoriatic arthritis"],"prefix":"10.1038","volume":"10","author":[{"given":"Dovile","family":"Sinkeviciute","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solveig","family":"Skovlund Groen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Manon-Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Aspberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrik","family":"\u00d6nnerfjord","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Christine","family":"Bay-Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salome","family":"Kristensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Signe","family":"Holm Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,11]]},"reference":[{"key":"70327_CR1","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1016\/j.jaad.2013.07.023","volume":"69","author":"PJ Mease","year":"2013","unstructured":"Mease, P. 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ACBJ holds stocks in Nordic Bioscience. Nordic Bioscience is a privately-owned, small\u2013medium size enterprise (SME) partly focused on the development of biomarkers. AA, SK and P\u00d6 have no competing interests. The study was supported by an industrial PhD scholarship grant (ID15-0035) from Swedish Strategic Research Foundation (SSF)\u2014recipients P\u00d6 and ACBJ. None of the authors received fees, bonuses or other benefits for the work described in the manuscript.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13541"}}