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This study sought to establish a sensitive and specific <jats:italic>Miox-NanoLuc<\/jats:italic> transgenic mouse for early detection of drug-induced nephrotoxicity. We generated <jats:italic>Miox-NanoLuc<\/jats:italic> transgenic mice with kidney-specific NanoLuc overexpression. Our data showed that Miox-NanoLuc-produced luminescence was kidney-specific and had good stability at room temperature, 4\u00a0\u00b0C,\u2009\u2212\u200920\u00a0\u00b0C, and repeated freeze\u2013thaw cycles. Serum levels of BUN and creatinine were significantly increased at day 2 or 3 in cisplatin-treated mice and at day 5 in aristolochic acid (AAI)-treated mice. Particularly, the serum and urine Miox-NanoLuc luminescence levels were significantly increased at day 1 in cisplatin-treated mice and at day 3 in AAI-treated mice. Renal pathological analysis showed that the kidney sections of cisplatin-treated mice at day 5 and AAI-treated mice at day 13 showed cytolysis and marked vacuolization of tubular cells. In conclusion, we developed a new platform to early quantify drug-induced nephrotoxicity before serum BUN and creatinine levels increased and pathological tubular cell injury occurred. This model may serve as an early detection for drug- and food-induced nephrotoxicity and as an animal model to investigate tubular cell injury.<\/jats:p>","DOI":"10.1038\/s41598-020-70502-3","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T12:27:09Z","timestamp":1597408029000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Kidney-based in vivo model for drug-induced nephrotoxicity testing"],"prefix":"10.1038","volume":"10","author":[{"given":"Yuan-Yow","family":"Chiou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si-Tse","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Sian","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hsuan","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,8,14]]},"reference":[{"key":"70502_CR1","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/s0140-6736(13)60687-x","volume":"382","author":"V Jha","year":"2013","unstructured":"Jha, V. et al. 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