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Urinary liporabinomannan (LAM) testing has been previously restricted to HIV co-infected TB patients. In this study we evaluate urinary LAM in HIV negative, pediatric and adult, pulmonary and extrapulmonary tuberculosis patients. We measured 430 microbiologically confirmed pretreatment tuberculosis patients and controls from Peru, Guinea Bissau, Venezuela, Uganda and the United States using three monoclonal antibodies, MoAb1, CS35, and A194, which recognize distinct LAM epitopes, a one-sided immunoassay, and blinded cohorts. We evaluated sources of assay variability and comorbidities (HIV and diabetes). All antibodies successfully discriminated TB positive from TB negative patients. ROAUC from the average of three antibodies\u2019 responses was 0.90; 95% CI 0.87\u20130.93, 90% sensitivity, 73.5% specificity (80\u00a0pg\/mL). MoAb1, recognizing the 5-methylthio-<jats:sc>d<\/jats:sc>-xylofuranose(MTX)-mannose(Man) cap epitope, performed the best, was less influenced by glycosuria and identified culture positive pediatric (N\u2009=\u200919) and extrapulmonary (N\u2009=\u200924) patients with high accuracy (ROAUC 0.87, 95% CI 0.77\u20130.98, 0.90 sensitivity 0.80 specificity at 80\u00a0pg\/mL; ROAUC\u2009=\u20090.96, 95% CI 0.92\u20130.99, 96% sensitivity, 80% specificity at 82\u00a0pg\/mL, respectively). The MoAb1 antibody, recognizing the MTX-Man cap epitope, is a novel analyte for active TB detection in pediatric and extrapulmonary disease.<\/jats:p>","DOI":"10.1038\/s41598-020-70669-9","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T10:02:46Z","timestamp":1597744966000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Lipoarabinomannan antigenic epitope differences in tuberculosis disease subtypes"],"prefix":"10.1038","volume":"10","author":[{"given":"Ruben","family":"Magni","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatlum","family":"Rruga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fahad 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Ceres Nanosciences (Nanotrap) licensed the technology from George Mason University Research Foundation. LAL, EFP and AL own shares of Ceres Nanosciences. B.L., B.K. are Ceres Nanosciences\u2019 employees who manufactured some reagents for this study. The other authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13944"}}