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Using BALB\/c mice, a filtration surgery model was made by incision of the limbal conjunctiva, followed by the insertion of a 33G needle tip into the anterior chamber, and 11-0 nylon sutures. Subgroups of mice were treated intraoperatively with 0.4\u00a0mg\/ml mitomycin-C (MMC). At day 3 after surgery the bleb was maintained. The bleb region tissue was sampled 3\u00a0days after the filtration surgery, and gene expression analysis was carried out using a mouse Agilent 8\u2009\u00d7\u200960\u00a0K array. We found 755 hyperexpressed transcripts in the bleb region compared to control conjunctiva. The hyperexpressed transcripts included epithelial cell metaplasia-related (<jats:italic>Il1b<\/jats:italic>, <jats:italic>Krt16<\/jats:italic>,<jats:italic> Sprr1b<\/jats:italic>), inflammation-related (<jats:italic>Ccl2<\/jats:italic>,<jats:italic> Il6<\/jats:italic>) and wound healing-related (<jats:italic>Lox<\/jats:italic>,<jats:italic> Timp1<\/jats:italic>) genes. We also found downregulation of a goblet cell marker gene (<jats:italic>Gp2<\/jats:italic>) in the bleb conjunctiva. MMC treatment suppressed elastin (<jats:italic>Eln<\/jats:italic>) gene expression and enhanced keratinization-related gene expression (<jats:italic>Krt1<\/jats:italic>,<jats:italic> Lor<\/jats:italic>) in the bleb region. Our results suggest the importance of epithelial wound healing after filtration surgery, and this filtration surgery model will be a useful tool for further pathophysiological analysis.<\/jats:p>","DOI":"10.1038\/s41598-020-72036-0","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T10:03:01Z","timestamp":1600077781000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Alteration of gene expression in mice after glaucoma filtration surgery"],"prefix":"10.1038","volume":"10","author":[{"given":"Keisuke","family":"Adachi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Asada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiaki","family":"Hirakata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Onoue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Iwamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshimitsu","family":"Kasuga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,9,14]]},"reference":[{"key":"72036_CR1","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1001\/jama.2014.3192","volume":"311","author":"RN Weinreb","year":"2014","unstructured":"Weinreb, R. 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