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Twenty-four hours after exposure, participants underwent bronchoscopy to collect epithelial cells whose DNA methylation was measured using the Illumina 450\u00a0K platform. We performed global and regional tests examining the ozone versus clean air effect on the DNA methylome and calculated Fisher-exact<jats:italic>p<\/jats:italic>-values for a series of univariate tests. We found little evidence of an overall effect of ozone on the DNA methylome but some suggestive changes in<jats:italic>PLSCR1<\/jats:italic>,<jats:italic>HCAR1<\/jats:italic>, and<jats:italic>LINC00336<\/jats:italic>DNA methylation after ozone exposure relative to clean air. 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