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By using the acute brainstem slice method and multi-electrode array system, we established a paraoxon (a typical acetylcholinesterase inhibitor) poisoning model to investigate the time-dependent changes in respiratory burst amplitudes of the pre-B\u00f6tzinger complex (respiratory rhythm generator). We then determined whether pralidoxime or atropine, which are antidotes of paraoxon, could counteract the effects of paraoxon. Herein, we showed that paraoxon significantly decreased the respiratory burst amplitude of the pre-B\u00f6tzinger complex (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Moreover, pralidoxime and atropine could suppress the decrease in amplitude by paraoxon (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Paraoxon directly impaired the pre-B\u00f6tzinger complex, and the findings implied that this impairment caused central apnoea or hypopnoea. Pralidoxime and atropine could therapeutically attenuate the impairment. This study is the first to prove the usefulness of the multi-electrode array method for electrophysiological and toxicological studies in the mammalian brainstem.<\/jats:p>","DOI":"10.1038\/s41598-020-73003-5","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T10:02:46Z","timestamp":1601287366000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Mechanism of central hypopnoea induced by organic phosphorus poisoning"],"prefix":"10.1038","volume":"10","author":[{"given":"Kazuhito","family":"Nomura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eichi","family":"Narimatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoko","family":"Kyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keigo","family":"Sawamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Uemura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuichiro","family":"Kakizaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Harada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"73003_CR1","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1126\/science.1683005","volume":"254","author":"JC Smith","year":"1991","unstructured":"Smith, J. 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Other authors have no conflict of interest, financial or otherwise for this study.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"15834"}}