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Although transgenic mice lacking the mitochondrial complex I accessory subunit NDUFS4 develop early-onset optic atrophy, severe systemic mitochondrial dysfunction leads to very early death and makes this mouse line impractical for studying the pathobiology of mitochondrial optic neuropathies. Theoretically, RGC-specific inactivation of <jats:italic>ndufs4<\/jats:italic> would allow characterization of RGC degeneration over a longer time course, provided that RGC death from mitochondrial dysfunction is a cell-autonomous process. We demonstrate that the vesicular glutamate transporter VGLUT2 may be exploited to drive robust Cre recombinase expression in RGCs without any expression observed in directly neighboring retinal cell types. Deletion of <jats:italic>ndufs4<\/jats:italic> in RGCs resulted in reduced expression of NDUFS4 protein within the optic nerves of <jats:italic>Vglut2-Cre;ndufs4<\/jats:italic><jats:sup><jats:italic>loxP\/loxP<\/jats:italic><\/jats:sup> mice. RGC degeneration in <jats:italic>Vglut2-Cre;ndufs4<\/jats:italic><jats:sup><jats:italic>loxP\/loxP<\/jats:italic><\/jats:sup> retinas commenced around postnatal day 45 (P45) and progressed to loss of two-thirds of RGCs by P90, confirming that intrinsic complex I dysfunction is sufficient to induce RGC death. The rapidly-developing optic atrophy makes the <jats:italic>Vglut2-Cre;ndufs4<\/jats:italic><jats:sup><jats:italic>loxP\/loxP<\/jats:italic><\/jats:sup> mouse line a promising preclinical model for testing therapies for currently untreatable mitochondrial optic neuropathies such as Leber Hereditary Optic Neuropathy.<\/jats:p>","DOI":"10.1038\/s41598-020-73353-0","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T10:05:01Z","timestamp":1601546701000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Progressive optic atrophy in a retinal ganglion cell-specific mouse model of complex I deficiency"],"prefix":"10.1038","volume":"10","author":[{"given":"Luyu","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikael","family":"Klingeborn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda M.","family":"Travis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim Y.","family":"Arshavsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Sidney M.","family":"Gospe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,1]]},"reference":[{"key":"73353_CR1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1167\/iovs.18-24289","volume":"59","author":"CR Fisher","year":"2018","unstructured":"Fisher, C. 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