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We investigated the presence of\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    in gastric cancer (GC) and gastric preneoplastic conditions of the stomach, and its potential prognostic value in GC patients.\n                    <jats:italic>Fusobacterium<\/jats:italic>\n                    spp. and\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    were quantified in various specimens from gastrointestinal tract including paired CRC and GC tissues using probe-based qPCR.\n                    <jats:italic>Fusobacterium<\/jats:italic>\n                    spp. and\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    were more frequently found in tumorous tissue of CRC and GC compared to non-tumorous tissues. The frequency and bacterial load were higher in CRC compared to GC patients.\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    positivity showed no association to chronic gastritis or preneoplastic conditions such as intestinal metaplasia.\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    -positivity was associated with significantly worse overall survival in patients with Lauren\u2019s diffuse type, but not with intestinal type GC. There was no association with gender,\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    -status, tumor stage or tumor localization. However,\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    was positively associated with patient\u2019s age and a trend for a lower global long interspersed element-1 DNA methylation. In conclusion, our work provides novel evidence for clinical relevance of\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    in GC by showing an association between\n                    <jats:italic>F. nucleatum<\/jats:italic>\n                    positivity with worse prognosis of patients with Laurens\u2019s diffuse type gastric cancer. Further studies are necessary to explore related mechanistic insights and potential therapeutic benefit of targeted antibiotic treatment in GC patients.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-73448-8","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T06:04:13Z","timestamp":1601532253000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["Fusobacterium nucleatum is associated with worse prognosis in Lauren\u2019s diffuse type gastric cancer patients"],"prefix":"10.1038","volume":"10","author":[{"given":"Ellen Teresa","family":"Boehm","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cosima","family":"Thon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juozas","family":"Kupcinskas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruta","family":"Steponaitiene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jurgita","family":"Skieceviciene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Canbay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Malfertheiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Link","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,1]]},"reference":[{"key":"73448_CR1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1126\/science.1224820","volume":"338","author":"JC Arthur","year":"2012","unstructured":"Arthur, J. 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