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The aim was to characterize and investigate molecular characteristics unique to <jats:italic>S. aureus<\/jats:italic> isolates from HHA associated prosthetic joint infections and potentially explain the HHA patients\u2019 elevated 1-year mortality compared to a non-HHA group. The comparison group consisted of patients with non-HHA lower-extremity implant-related <jats:italic>S. aureus<\/jats:italic> infections. <jats:italic>S. aureus<\/jats:italic> isolates from diagnostic patient samples were whole-genome sequenced. Univariate and multivariate analyses were performed to detect group-associated genetic signatures. A total of 62 HHA patients and 73 non-HHA patients were included. Median age (81\u00a0years vs. 74\u00a0years; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and 1-year mortality (44% vs. 15%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) were higher in the HHA group. A total of 20 clonal clusters (CCs) were identified; 75% of the isolates consisted of CC45, CC30, CC5, CC15, and CC1. Analyses of core and accessory genome content, including virulence, resistance genes, and k-mer analysis revealed few group-associated variants, none of which could explain the elevated 1-year mortality in HHA patients. Our findings support the premise that all <jats:italic>S. aureus<\/jats:italic> can cause invasive infections given the opportunity.<\/jats:p>","DOI":"10.1038\/s41598-020-73736-3","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T10:04:06Z","timestamp":1601978646000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular characteristics of Staphylococcus aureus associated prosthetic joint infections after hip fractures treated with hemiarthroplasty: a retrospective genome-wide association study"],"prefix":"10.1038","volume":"10","author":[{"given":"J. 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