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The prevalence of DWV in honey bees has led to significant concerns about spillover of the virus to other pollinating species. Bumble bees are both a major group of wild and commercially-reared pollinators. Several studies have reported pathogen spillover of DWV from honey bees to bumble bees, but evidence of a sustained viral infection characterized by virus replication and accumulation has yet to be demonstrated. Here we investigate the infectivity and transmission of DWV in bumble bees using the buff-tailed bumble bee\n                    <jats:italic>Bombus terrestris<\/jats:italic>\n                    as a model. We apply a reverse genetics approach combined with controlled laboratory conditions to detect and monitor DWV infection. A novel reverse genetics system for three representative DWV variants, including the two master variants of DWV\u2014type A and B\u2014was used. Our results directly confirm DWV replication in bumble bees but also demonstrate striking resistance to infection by certain transmission routes. Bumble bees may support DWV replication but it is not clear how infection could occur under natural environmental conditions.\n                  <\/jats:p>","DOI":"10.1038\/s41598-020-73809-3","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T06:03:22Z","timestamp":1602137002000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Evidence for and against deformed wing virus spillover from honey bees to bumble bees: a reverse genetic analysis"],"prefix":"10.1038","volume":"10","author":[{"given":"Olesya N.","family":"Gusachenko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Woodford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharin","family":"Balbirnie-Cumming","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene V.","family":"Ryabov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,8]]},"reference":[{"key":"73809_CR1","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1126\/science.1220941","volume":"336","author":"SJ Martin","year":"2012","unstructured":"Martin, S. 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