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Rapid identification and novel approaches for the characterization of these fungi are of great interest to improve the diagnosis and the knowledge about their pathogenic properties. This study aimed to characterize clinical isolates of <jats:italic>Candida<\/jats:italic> spp. by proteomics (MALDI-TOF MS) and metabolomics (<jats:sup>1<\/jats:sup>H-NMR), and to correlate their metabolic profiles with <jats:italic>Candida<\/jats:italic> species, source of infection and different virulence associated parameters. In particular, 49 <jats:italic>Candida<\/jats:italic> strains from different sources (blood, n\u2009=\u200915; vagina, n\u2009=\u200918; respiratory tract, n\u2009=\u200916), belonging mainly to <jats:italic>C. albicans<\/jats:italic> complex (61%), <jats:italic>C. glabrata<\/jats:italic> (20%) and <jats:italic>C. parapsilosis<\/jats:italic> (12%) species were used. Several extracellular and intracellular metabolites showed significantly different concentrations among isolates recovered from different sources of infection, as well as among different <jats:italic>Candida<\/jats:italic> species. These metabolites were mainly related to the glycolysis or gluconeogenesis, tricarboxylic acid cycle, nucleic acid synthesis and amino acid and lipid metabolism. Moreover, we found specific metabolic fingerprints associated with the ability to form biofilm, the antifungal resistance (i.e. caspofungin and fluconazole) and the production of secreted aspartyl proteinase. In conclusion, <jats:sup>1<\/jats:sup>H-NMR-based metabolomics can be useful to deepen <jats:italic>Candida<\/jats:italic> spp. virulence and pathogenicity properties.<\/jats:p>","DOI":"10.1038\/s41598-020-73889-1","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T10:02:46Z","timestamp":1602064966000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Metabolic profiling of Candida clinical isolates of different species and infection sources"],"prefix":"10.1038","volume":"10","author":[{"given":"Josidel Concei\u00e7\u00e3o","family":"Oliver","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Laghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carola","family":"Parolin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Foschi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Marangoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Liberatore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Latercia Tranches","family":"Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Cricca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Vitali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,7]]},"reference":[{"key":"73889_CR1","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1099\/jmm.0.045054-0","volume":"62","author":"JCO Sardi","year":"2013","unstructured":"Sardi, J. 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