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Cell wall growth is facilitated by peptidoglycan synthases and hydrolases and is potentially modulated by components of the central carbon metabolism. In <jats:italic>Bacillus subtilis,<\/jats:italic> UgtP synthesises the glucolipid precursor for lipoteichoic acid and has been suggested to function as a metabolic sensor governing cell size. Here we show that <jats:italic>ugtP<\/jats:italic> mutant cells have increased levels of cell wall precursors and changes in their peptidoglycan that suggest elevated <jats:sc>dl<\/jats:sc>-endopeptidase activity. The additional deletion of <jats:italic>lytE<\/jats:italic>, encoding a <jats:sc>dl<\/jats:sc>-endopeptidase important for cell elongation, in the <jats:italic>ugtP<\/jats:italic> mutant background produced cells with severe shape defects. Interestingly, the <jats:italic>ugtP lytE<\/jats:italic> mutant recovered normal rod-shape by acquiring mutations that decreased the expression of the peptidoglycan synthase PBP1. Together our results suggest that cells lacking <jats:italic>ugtP<\/jats:italic> must re-adjust the balance between peptidoglycan synthesis and hydrolysis to maintain proper cell morphology.<\/jats:p>","DOI":"10.1038\/s41598-020-74609-5","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T10:04:15Z","timestamp":1603274655000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Cell morphology maintenance in Bacillus subtilis through balanced peptidoglycan synthesis and hydrolysis"],"prefix":"10.1038","volume":"10","author":[{"given":"Jad","family":"Sassine","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Lalk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Daniel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waldemar","family":"Vollmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,21]]},"reference":[{"key":"74609_CR1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1574-6976.2007.00094.x","volume":"32","author":"W Vollmer","year":"2008","unstructured":"Vollmer, W., Blanot, D. & de Pedro, M. 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