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Using sterile fish in commercial aquaculture operations is, therefore, a sustainable strategy for bio-containment. So far, the only commercially used methodology for producing sterile fish is triploidization. However, triploid fish are less robust. A novel approach in which to achieve sterility is to produce germ cell-free salmon, which can be accomplished by knocking out the <jats:italic>dead-end<\/jats:italic> (<jats:italic>dnd<\/jats:italic>) gene using CRISPR-Cas9. The lack of germ cells in the resulting <jats:italic>dnd<\/jats:italic> crispants, thus, prevents reproduction and inhibits subsequent large-scale production of sterile fish. Here, we report a rescue approach for producing germ cells in Atlantic salmon <jats:italic>dnd<\/jats:italic> crispants. To achieve this, we co-injected the wild-type (wt) variant of salmon <jats:italic>dnd<\/jats:italic> mRNA together with CRISPR-Cas9 constructs targeting <jats:italic>dnd<\/jats:italic> into 1-cell stage embryos. We found that rescued one-year-old fish contained germ cells, type A spermatogonia in males and previtellogenic primary oocytes in females. The method presented here opens a possibility for large-scale production of germ-cell free Atlantic salmon offspring through the genetically sterile broodstock which can pass the sterility trait on the next generation.<\/jats:p>","DOI":"10.1038\/s41598-020-74876-2","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T10:03:14Z","timestamp":1603360994000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon"],"prefix":"10.1038","volume":"10","author":[{"given":"Hilal","family":"G\u00fcralp","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai O.","family":"Skaftnesmo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Kj\u00e6rner-Semb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Hege","family":"Straume","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Kleppe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00fcdiger W.","family":"Schulz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf B.","family":"Edvardsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Wargelius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,10,22]]},"reference":[{"key":"74876_CR1","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1093\/icesjms\/fsu132","volume":"72","author":"GL Taranger","year":"2015","unstructured":"Taranger, G. 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